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Mevastatin-Induced AP-1-Dependent HO-1 Expression Suppresses Vascular Cell Adhesion Molecule-1 Expression and Monocyte Adhesion on Human Pulmonary Alveolar Epithelial Cells Challenged with TNF-α

Authors :
Chien-Chung Yang
Rou-Ling Cho
Chuen-Mao Yang
Li-Der Hsiao
Chih-Chung Lin
Source :
Biomolecules, Vol 10, Iss 3, p 381 (2020), Biomolecules, Volume 10, Issue 3
Publication Year :
2020
Publisher :
MDPI AG, 2020.

Abstract

Mevastatin (MVS) has been previously shown to induce heme oxygenase (HO)-1 expression through Nox/ROS-dependent PDGFR&alpha<br />/PI3K/Akt/Nrf2/ARE axis in human pulmonary alveolar epithelial cells (HPAEpiCs). However, alternative signaling pathways might involve in MVS-induced HO-1 expression. We found that tumor necrosis factor &alpha<br />(TNF&alpha<br />) induced vascular cell adhesion protein 1 (VCAM-1) expression and NF-&kappa<br />B p65 phosphorylation which were attenuated by pretreatment with MVS via up-regulation of HO-1, determined by Western blot and real-time qPCR. TNF&alpha<br />induced VCAM-1 expression was attenuated by an NF-&kappa<br />B inhibitor, Bay117082. The inhibitory effects of MVS were reversed by tin protoporphyrin (SnPP)IX (an inhibitor of HO-1 activity). In addition, pretreatment with the inhibitor of pan-Protein kinase C (PKC) (GF109203X), PKC&alpha<br />(G&ouml<br />6983), Pyk2 (PF431396), p38&alpha<br />MAPK (SB202190), JNK1/2 (SP600125), or AP-1 (Tanshinone IIA), and transfection with their respective siRNAs abolished MVS-induced HO-1 expression in HPAEpiCs. c-Jun (one of AP-1 subunits) was activated by PKC&alpha<br />Pyk2, p38&alpha<br />MAPK, and JNK1/2, which turned on the transcription of the homx1 gene. The interaction between c-Jun and HO-1 promoter was confirmed by a chromatin immunoprecipitation (ChIP) assay, which was attenuated by these pharmacological inhibitors. These results suggested that MVS induces AP-1/HO-1 expression via PKC&alpha<br />/Pyk2/p38&alpha<br />MAPK- or JNK1/2-dependent c-Jun activation, which further binds with AP-1-binding site on HO-1 promoter and suppresses the TNF&alpha<br />mediated inflammatory responses in HPAEpiCs. Thus, upregulation of the AP-1/HO-1 system by MVS exerts a potentially therapeutic strategy to protect against pulmonary inflammation.

Details

Language :
English
Volume :
10
Issue :
3
Database :
OpenAIRE
Journal :
Biomolecules
Accession number :
edsair.doi.dedup.....1b690bebc7f2537dc05a46de17aba4f5