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Inhibition of Transforming Growth Factor β (TGF-β) Signaling can Substitute for Oct4 Protein in Reprogramming and Maintain Pluripotency*
- Publication Year :
- 2014
- Publisher :
- American Society for Biochemistry and Molecular Biology, 2014.
-
Abstract
- Mouse pluripotent stem cells (PSCs), such as ES cells and induced PSCs (iPSCs), are an excellent system to investigate the molecular and cellular mechanisms involved in early embryonic development. The signaling pathways orchestrated by leukemia inhibitor factor/STAT3, Wnt/β-catenin, and FGF/MEK/ERK play key roles in the generation of pluripotency. However, the function of TGF-β signaling in this process remains elusive. Here we show that inhibiting TGF-β signaling with its inhibitor SB431542 can substitute for Oct4 during reprogramming. Moreover, inhibiting TGF-β signaling can sustain the pluripotency of iPSCs and ES cells through modulating FGF/MEK/ERK signaling. Therefore, this study reveals a novel function of TGF-β signaling inhibition in the generation and maintenance of PSCs.
- Subjects :
- MAPK/ERK pathway
animal structures
Cellular differentiation
Blotting, Western
Induced Pluripotent Stem Cells
MAP Kinase Kinase 1
Fluorescent Antibody Technique
Dioxoles
Fibroblast growth factor
Real-Time Polymerase Chain Reaction
Biochemistry
Mice
Transforming Growth Factor beta
Animals
RNA, Messenger
Induced pluripotent stem cell
Extracellular Signal-Regulated MAP Kinases
Molecular Biology
Cells, Cultured
Embryonic Stem Cells
biology
Reverse Transcriptase Polymerase Chain Reaction
Wnt signaling pathway
Cell Differentiation
Transforming growth factor beta
Cell Biology
Fibroblasts
Cellular Reprogramming
Embryo, Mammalian
Embryonic stem cell
Cell biology
Mice, Inbred C57BL
embryonic structures
Benzamides
biology.protein
Fibroblast Growth Factor 1
Reprogramming
Octamer Transcription Factor-3
Signal Transduction
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....1baa555ef3d0c9071b88e975e71fcb2b