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Syndecan-4 is regulated by IL-1β in β-cells and human islets
- Source :
- Molecular and Cellular Endocrinology, Vol. 510 (2020) P. 110815
- Publication Year :
- 2020
-
Abstract
- Syndecans (SDC) are important multifunctional components of the extracellular matrix mainly described in endothelial cells. We studied the expression and regulation of SDC in cultured MIN6B1 cells and pancreatic islets. qRT-PCR revealed that syndecan-4 (SDC4) was the predominant isoform expressed in MIN6B1 cells and islets compared to other forms of SDC. Immunofluorescence in mouse and human pancreas sections revealed that SDC4 is mainly expressed in β-cells compared to other pancreatic cells. Exposure of MIN6B1 and human islets to IL-1β dose-dependently induced a rapid and transient expression of SDC4 while SRC and STAT3 inhibitors decreased this effect. Exposure of human islets to Il-1β caused an increase of SDC4 shedding, however treatment with STAT3 and SRC inhibitors inhibited this effect. These results indicate that SDC4 is upregulated by IL-1β through the SRC-STAT3 pathway and this pathway is also involved in SDC4 shedding in islets.
- Subjects :
- 0301 basic medicine
Gene isoform
Male
STAT3 Transcription Factor
Interleukin-1beta
030209 endocrinology & metabolism
Immunofluorescence
Biochemistry
Syndecan 1
STAT3
Extracellular matrix
03 medical and health sciences
0302 clinical medicine
Endocrinology
Downregulation and upregulation
Insulin-Secreting Cells
medicine
Animals
Humans
Protein Isoforms
RNA, Messenger
Molecular Biology
Human islet
ddc:617
biology
medicine.diagnostic_test
Chemistry
Pancreatic islets
Diabetes
Cell Membrane
β-cell
Cell biology
Up-Regulation
Mice, Inbred C57BL
Protein Transport
030104 developmental biology
medicine.anatomical_structure
src-Family Kinases
Matrix Metalloproteinase 9
IL-1β
biology.protein
Syndecan-4
SRC
Proto-oncogene tyrosine-protein kinase Src
Signal Transduction
Subjects
Details
- ISSN :
- 18728057 and 03037207
- Volume :
- 510
- Database :
- OpenAIRE
- Journal :
- Molecular and cellular endocrinology
- Accession number :
- edsair.doi.dedup.....1bc2a8c861e518a263d412e6d84cbe55