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Genome-Wide Expression of MicroRNAs Is Regulated by DNA Methylation in Hepatocarcinogenesis
- Source :
- Gastroenterology Research and Practice, Vol 2015 (2015), Gastroenterology Research and Practice
- Publication Year :
- 2015
- Publisher :
- Hindawi Limited, 2015.
-
Abstract
- Background.Previous studies, including ours, have examined the regulation of microRNAs (miRNAs) by DNA methylation, but whether this regulation occurs at a genome-wide level in hepatocellular carcinoma (HCC) is unclear.Subjects/Methods.Using a two-phase study design, we conducted genome-wide screening for DNA methylation and miRNA expression to explore the potential role of methylation alterations in miRNAs regulation.Results.We found that expressions of 25 miRNAs were statistically significantly different between tumor and nontumor tissues and perfectly differentiated HCC tumor from nontumor. Six miRNAs were overexpressed, and 19 were repressed in tumors. Among 133 miRNAs with inverse correlations between methylation and expression, 8 miRNAs (6%) showed statistically significant differences in expression between tumor and nontumor tissues. Six miRNAs were validated in 56 additional paired HCC tissues, and significant inverse correlations were observed for miR-125b and miR-199a, which is consistent with the inactive chromatin pattern found in HepG2 cells.Conclusion.These data suggest that the expressions of miR-125b and miR-199a are dramatically regulated by DNA hypermethylation that plays a key role in hepatocarcinogenesis.
- Subjects :
- Article Subject
Hepatology
Gastroenterology
Methylation
Biology
Bioinformatics
medicine.disease
Chromatin
Mirna expression
Hepg2 cells
Hepatocellular carcinoma
DNA methylation
microRNA
Cancer research
medicine
lcsh:Diseases of the digestive system. Gastroenterology
lcsh:RC799-869
Genome wide expression
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 16876121
- Volume :
- 2015
- Database :
- OpenAIRE
- Journal :
- Gastroenterology Research and Practice
- Accession number :
- edsair.doi.dedup.....1bd0bf198deb166fdc9c0783666733a2