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The Aryl Hydrocarbon Receptor Pathway Defines the Time Frame for Restorative Neurogenesis
- Source :
- Cell reports 25(12), 3241-3251.e5 (2018). doi:10.1016/j.celrep.2018.11.055, Cell Reports, Vol 25, Iss 12, Pp 3241-3251.e5 (2018), Cell Rep. 25, 3241-3251.e5 (2018)
- Publication Year :
- 2018
- Publisher :
- Elsevier, 2018.
-
Abstract
- Summary: Zebrafish have a high capacity to replace lost neurons after brain injury. New neurons involved in repair are generated by a specific set of glial cells, known as ependymoglial cells. We analyze changes in the transcriptome of ependymoglial cells and their progeny after injury to infer the molecular pathways governing restorative neurogenesis. We identify the aryl hydrocarbon receptor (AhR) as a regulator of ependymoglia differentiation toward post-mitotic neurons. In vivo imaging shows that high AhR signaling promotes the direct conversion of a specific subset of ependymoglia into post-mitotic neurons, while low AhR signaling promotes ependymoglial proliferation. Interestingly, we observe the inactivation of AhR signaling shortly after injury followed by a return to the basal levels 7 days post injury. Interference with timely AhR regulation after injury leads to aberrant restorative neurogenesis. Taken together, we identify AhR signaling as a crucial regulator of restorative neurogenesis timing in the zebrafish brain. : Zebrafish have a high capacity to replace lost neurons after brain injury. Di Giaimo et al. identify the aryl hydrocarbon receptor (AhR) as a crucial regulator of restorative neurogenesis timing in the zebrafish brain. Interference with timely AhR regulation after injury leads to aberrant restorative neurogenesis. Keywords: neurogenesis, regeneration, aryl hydrocarbon receptor, direct conversion, zebrafish, live imaging
- Subjects :
- 0301 basic medicine
Time Factors
Cell Survival
Neurogenesis
Ependymoglial Cells
neurogenesi
Regulator
cytology [Ependymoglial Cells]
Mitosis
Biology
General Biochemistry, Genetics and Molecular Biology
Transcriptome
03 medical and health sciences
Live cell imaging
Animals
direct conversion
ddc:610
Zebrafish
lcsh:QH301-705.5
Cell Proliferation
Neurons
metabolism [Ependymoglial Cells]
Biochemistry, Genetics and Molecular Biology (all)
aryl hydrocarbon receptor
Aryl Hydrocarbon Receptor
Direct Conversion
Live Imaging
Regeneration
Regeneration (biology)
metabolism [Receptors, Aryl Hydrocarbon]
Cell Differentiation
live imaging
zebrafish
Aryl hydrocarbon receptor
biology.organism_classification
Cell biology
030104 developmental biology
lcsh:Biology (General)
Receptors, Aryl Hydrocarbon
regeneration
cytology [Neurons]
biology.protein
Signal Transduction
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Cell reports 25(12), 3241-3251.e5 (2018). doi:10.1016/j.celrep.2018.11.055, Cell Reports, Vol 25, Iss 12, Pp 3241-3251.e5 (2018), Cell Rep. 25, 3241-3251.e5 (2018)
- Accession number :
- edsair.doi.dedup.....1be3a3e2013a7a26fccaaca42a8b76ab