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Insight into nephrocan function in mouse endoderm patterning
- Source :
- International Journal of Molecular Sciences, International journal of molecular sciences, 21 (2019). doi:10.3390/ijms21010008, info:cnr-pdr/source/autori:Addeo, Martina; Buonaiuto, Silvia; Guerriero, Ilaria; Amendola, Elena; Visconte, Feliciano; Marino, Antonio; De Angelis, Maria Teresa; Russo, Filomena; Roberto, Luca; Marotta, Pina; Antonino Russo, Nicola; Iervolino, Anna; Amodio, Federica; De Felice, Mario; Lucci, Valeria; Falco, Geppino/titolo:Insight into nephrocan function in mouse endoderm patterning/doi:10.3390%2Fijms21010008/rivista:International journal of molecular sciences (Print)/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume:21, Volume 21, Issue 1
- Publication Year :
- 2020
-
Abstract
- Endoderm-derived organs as liver and pancreas are potential targets for regenerative therapies, and thus, there is great interest in understanding the pathways that regulate the induction and specification of this germ layer. Currently, the knowledge of molecular mechanisms that guide the in vivo endoderm specification is restricted by the lack of early endoderm specific markers. Nephrocan (Nepn) is a gene whose expression characterizes the early stages of murine endoderm specification (E7.5&ndash<br />11.5) and encodes a secreted N-glycosylated protein. In the present study, we report the identification of a new transcript variant that is generated through alternative splicing. The new variant was found to have differential and tissue specific expression in the adult mouse. In order to better understand Nepn role during endoderm specification, we generated Nepn knock-out (KO) mice. Nepn&minus<br />/&minus<br />mice were born at Mendelian ratios and displayed no evident phenotype compared to WT mice. In addition, we produced nullizygous mouse embryonic stem cell (mESC) line lacking Nepn by applying (CRISPR)/CRISPR-associated systems 9 (Cas9) and employed a differentiation protocol toward endoderm lineage. Our in vitro results revealed that Nepn loss affects the endoderm differentiation impairing the expression of posterior foregut-associated markers.
- Subjects :
- 0301 basic medicine
Nephrocan gene
Mice
0302 clinical medicine
Intercellular Signaling Peptides and Protein
CRISPR
Protein Isoforms
Spectroscopy
Gene Editing
Mice, Knockout
differentiation, definitive endoderm
Endoderm
Gene Expression Regulation, Developmental
Cell Differentiation
General Medicine
embryonic stem cells
Phenotype
Computer Science Applications
Cell biology
medicine.anatomical_structure
030220 oncology & carcinogenesis
Differentiation
embryonic structures
Gene Targeting
Intercellular Signaling Peptides and Proteins
Transcriptional variant
(CRISPR)/CRISPR-associated systems 9 (Cas9)
animal structures
Germ layer
[object Object]
Biology
Catalysis
Article
Mouse model
Inorganic Chemistry
03 medical and health sciences
medicine
Definitive endoderm
Animals
Physical and Theoretical Chemistry
Molecular Biology
Gene
Body Patterning
transcriptional variants
Animal
Organic Chemistry
Alternative splicing
Embryonic stem cell
030104 developmental biology
Genetic Loci
Function (biology)
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences, International journal of molecular sciences, 21 (2019). doi:10.3390/ijms21010008, info:cnr-pdr/source/autori:Addeo, Martina; Buonaiuto, Silvia; Guerriero, Ilaria; Amendola, Elena; Visconte, Feliciano; Marino, Antonio; De Angelis, Maria Teresa; Russo, Filomena; Roberto, Luca; Marotta, Pina; Antonino Russo, Nicola; Iervolino, Anna; Amodio, Federica; De Felice, Mario; Lucci, Valeria; Falco, Geppino/titolo:Insight into nephrocan function in mouse endoderm patterning/doi:10.3390%2Fijms21010008/rivista:International journal of molecular sciences (Print)/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume:21, Volume 21, Issue 1
- Accession number :
- edsair.doi.dedup.....1be78f97dc11d90f812861748f65f17e