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Enhanced effect of modified Zika virus E antigen on the immunogenicity of DNA vaccine
- Source :
- Virology. 549:25-31
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- It has been reported worldwide that the Zika virus (ZIKV) could be transmitted through placentas and sexual contact. ZIKV can also cause Guillain-Barre syndrome, microcephaly and neurological abnormalities. However, there are no approved vaccines available. We constructed six DNA vaccine candidates and tested the immunogenicity. Tandem repeated envelope domain Ⅲ (ED Ⅲ × 3) induced highly total IgG and neutralization antibody, as well as CD8+ T cell responses. Also, stem region-removed envelope (E ΔSTEM) elicited a robust production of IFN-γ in mice. To examine in vivo protection, we used mice treated with an IFNAR1 blocking antibody before and after the challenge. Vaccination with the two candidates led to a decline in the level of viral RNAs in organs. Moreover, the sera from the vaccinated mice did not enhance the infection of Dengue virus in K562 cells. These findings suggest the potential for the development of a novel ZIKV DNA vaccine.
- Subjects :
- Receptor, Interferon alpha-beta
Dengue virus
Antibodies, Viral
medicine.disease_cause
Zika virus
DNA vaccination
Mice
03 medical and health sciences
Immunogenicity, Vaccine
Viral Envelope Proteins
Antigen
Virology
Chlorocebus aethiops
Blocking antibody
Vaccines, DNA
medicine
Animals
Humans
Antigens, Viral
Vero Cells
030304 developmental biology
0303 health sciences
biology
Zika Virus Infection
Immunogenicity
Vaccination
030302 biochemistry & molecular biology
Viral Vaccines
Zika Virus
Dengue Virus
biology.organism_classification
Antibodies, Neutralizing
Disease Models, Animal
HEK293 Cells
biology.protein
Female
Antibody
K562 Cells
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 549
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....1bf3a08184ba280bdf85012bcd342dc3
- Full Text :
- https://doi.org/10.1016/j.virol.2020.07.014