Back to Search
Start Over
Aberrant Scinderin Expression Correlates With Liver Metastasis and Poor Prognosis in Colorectal Cancer
- Source :
- Frontiers in Pharmacology, Frontiers in Pharmacology, Vol 10 (2019)
- Publication Year :
- 2019
- Publisher :
- Frontiers Media S.A., 2019.
-
Abstract
- Many genes and mutations have been reported for colorectal cancer (CRC); however, very few have been associated with colorectal cancer liver metastasis (CRLM). We performed gene expression profiling experiments to identify genetic markers for CRLM and elucidate the molecular mechanisms. Microarray experiments were performed on CRC primary tumor samples with or without liver metastasis (LM) using the Affymetrix U133 plus 2.0 GeneChip Array. A new identified gene-scinderin (SCIN) was overexpressed with synchronous LM at both the RNA level evaluated with quantitative real-time PCR and protein level evaluated with immunohistochemistry and also with short overall survival analyzed with Kaplan-Meier method. With multivariate analysis indicated that SCIN served as an independent poor prognostic predictor for CRC patients. Disease-free survival was also significantly lower in SCIN overexpressing CRC patients with metachronous LM. In addition, SCIN knockdown significantly reduced cell proliferation, induced cell cycle arrest, and promoted the expression of some cell cycle apoptosis-related protein. Moreover, the DIAPH1, STAT3, CDK2, CDK4, and EGFR levels were downregulated, whereas CDKN2B and COL4A1 were upregulated in DLD-1-shSCIN cells by microarray analysis compared with DLD-1 shCon cells. These findings revealed that SCIN may serve as an important predictor of CRLM and poor outcome for CRC patients. SCIN may be a potential therapeutic target in human CRC. However, translation of its roles into clinical practice will require further investigation and additional experimental validation.
- Subjects :
- 0301 basic medicine
Microarray
Colorectal cancer
colorectal cancer
Metastasis
03 medical and health sciences
0302 clinical medicine
CDKN2B
medicine
Pharmacology (medical)
Original Research
Pharmacology
scinderin
genome-wide association study
Microarray analysis techniques
business.industry
lcsh:RM1-950
Cell cycle
medicine.disease
Primary tumor
Gene expression profiling
liver metastasis
lcsh:Therapeutics. Pharmacology
030104 developmental biology
030220 oncology & carcinogenesis
Cancer research
prognosis
business
Subjects
Details
- Language :
- English
- ISSN :
- 16639812
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Frontiers in Pharmacology
- Accession number :
- edsair.doi.dedup.....1bf9e952c94a0ef522bd13f3808235f9