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Basic fibroblast growth factor promotes macaque follicle development in vitro
- Source :
- REPRODUCTION. 149:425-433
- Publication Year :
- 2015
- Publisher :
- Bioscientifica, 2015.
-
Abstract
- Fertility preservation is an important type of frontier scientific research in the field of reproductive health. The culture of ovarian cortices to i) initiate primordial follicle growth and ii) procure developing follicles for later oocyte maturation is a promising fertility preservation strategy, especially for older women or cancer patients. At present, this goal remains largely unsubstantiated in primates because of the difficulty in attaining relatively large follicles via ovarian cortex culture. To overcome this hurdle, we cultured macaque monkey ovarian cortices with FSH, kit ligand (KL), basic fibroblast growth factor (bFGF), and/or epidermal growth factor (EGF). The various factors and factor combinations promoted primordial follicle development to different extents. Notably, both bFF (bFGF, 100 ng/ml and FSH, 50 ng/ml) and KF (KL, 100 ng/ml and FSH, 50 ng/ml) contributed to the activation of primordial follicles at day 12 (D12) of culture, whereas at D18, the proportions of developing follicles were significantly higher in the bFF and KF groups relative to the other treatment groups, particularly in the bFF group. Estradiol and progesterone production were also highest in the bFF group, and primary follicle diameters were the largest. Up until D24, the bFF group still exhibited the highest proportion of developing follicles. In conclusion, the bFGF–FSH combination promotes nonhuman primate primordial follicle developmentin vitro, with the optimal experimental window within 18 days. These results provide evidence for the future success of human ovarian cortex culture and the eventual acquisition of mature human follicles or oocytes for fertility restoration.
- Subjects :
- Embryology
Ovarian Cortex
Basic fibroblast growth factor
Radioimmunoassay
In Vitro Techniques
Biology
Andrology
Follicle
chemistry.chemical_compound
Endocrinology
Ovarian Follicle
Epidermal growth factor
medicine
Animals
Humans
Fertility preservation
Epidermal Growth Factor
Fibroblast growth factor receptor 2
Obstetrics and Gynecology
Cell Biology
Oocyte
Hormones
medicine.anatomical_structure
Reproductive Medicine
chemistry
Macaca
Female
Fibroblast Growth Factor 2
Folliculogenesis
Follicle Stimulating Hormone
Subjects
Details
- ISSN :
- 17417899 and 14701626
- Volume :
- 149
- Database :
- OpenAIRE
- Journal :
- REPRODUCTION
- Accession number :
- edsair.doi.dedup.....1c0bd2318d64c65839a26defbae16aaf