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Basic fibroblast growth factor promotes macaque follicle development in vitro

Authors :
Ting Ding
Jie Qiao
Jiangman Gao
Rong Li
Liying Yan
Tianren Wang
Yang Yu
Xu Zhi
Jie Yan
Xi Xia
Cuiling Lu
Source :
REPRODUCTION. 149:425-433
Publication Year :
2015
Publisher :
Bioscientifica, 2015.

Abstract

Fertility preservation is an important type of frontier scientific research in the field of reproductive health. The culture of ovarian cortices to i) initiate primordial follicle growth and ii) procure developing follicles for later oocyte maturation is a promising fertility preservation strategy, especially for older women or cancer patients. At present, this goal remains largely unsubstantiated in primates because of the difficulty in attaining relatively large follicles via ovarian cortex culture. To overcome this hurdle, we cultured macaque monkey ovarian cortices with FSH, kit ligand (KL), basic fibroblast growth factor (bFGF), and/or epidermal growth factor (EGF). The various factors and factor combinations promoted primordial follicle development to different extents. Notably, both bFF (bFGF, 100 ng/ml and FSH, 50 ng/ml) and KF (KL, 100 ng/ml and FSH, 50 ng/ml) contributed to the activation of primordial follicles at day 12 (D12) of culture, whereas at D18, the proportions of developing follicles were significantly higher in the bFF and KF groups relative to the other treatment groups, particularly in the bFF group. Estradiol and progesterone production were also highest in the bFF group, and primary follicle diameters were the largest. Up until D24, the bFF group still exhibited the highest proportion of developing follicles. In conclusion, the bFGF–FSH combination promotes nonhuman primate primordial follicle developmentin vitro, with the optimal experimental window within 18 days. These results provide evidence for the future success of human ovarian cortex culture and the eventual acquisition of mature human follicles or oocytes for fertility restoration.

Details

ISSN :
17417899 and 14701626
Volume :
149
Database :
OpenAIRE
Journal :
REPRODUCTION
Accession number :
edsair.doi.dedup.....1c0bd2318d64c65839a26defbae16aaf