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Coffee Restores Expression of lncRNAs Involved in Steatosis and Fibrosis in a Mouse Model of NAFLD
- Source :
- Nutrients, Vol 13, Iss 2952, p 2952 (2021), Nutrients, Nutrients; Volume 13; Issue 9; Pages: 2952
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- Background and aim: Coffee intake exerts protective effects against non-alcoholic fatty liver disease (NAFLD), although without fully cleared mechanisms. In this study we aimed to assess whether coffee consumption may influence the expression of long non-coding RNAs (lncRNAs) in the liver. Methods: C57BL/6J mice were fed a 12-week standard diet (SD), high-fat diet (HFD) or HFD plus decaffeinated coffee solution (HFD + coffee). Expression of specific lncRNAs involved in NAFLD was analyzed by real-time PCR. For the most differentially expressed lncRNAs, the analysis was also extended to their mRNA targets. Results: Decaffeinated coffee intake reduced body weight gain, prevented NAFLD, lowered hyperglycemia and hypercholesterolemia. NAFLD was associated with lower hepatic expression of Gm16551, a lncRNA inhibiting de novo lipogenesis, and higher expression of H19, a lncRNA promoting fibrogenesis. Coffee intake restored Gm16551 to levels observed in lean mice and downregulated gene expression of its targets acetyl coenzyme A carboxylase 1 and stearoyl coenzyme A desaturase 1. Furthermore, coffee consumption markedly decreased hepatic expression of H19 and of its target gene collagen alpha-1(I) chain; consistently, in mice fed HFD + coffee liver expression of αSMA protein returned to levels of mice fed SD. Expression of lncRNA involved in circadian clock such as fatty liver-related lncRNA 1 (FLRL1) and fatty liver-related lncRNA 2 (FLRL2) were upregulated by HFD and were also modulated by coffee intake. Conclusion. Hepatoprotective effects of coffee may be depending on the modulation of lncRNAs involved in key pathways of NAFLD onset and progression.
- Subjects :
- lncRNA
Liver Cirrhosis
Male
medicine.medical_specialty
Gm16551
H19
NAFLD
coffee
Animals
Coffee
Disease Models, Animal
Fatty Liver
Gene Expression
Liver
Mice
Mice, Inbred C57BL
Non-alcoholic Fatty Liver Disease
RNA, Long Noncoding
Coenzyme A
Circadian clock
Biology
Inbred C57BL
Article
chemistry.chemical_compound
Downregulation and upregulation
Fibrosis
Settore BIO/13 - Biologia Applicata
Internal medicine
medicine
TX341-641
Messenger RNA
Nutrition and Dietetics
Nutrition. Foods and food supply
Animal
Fatty liver
nutritional and metabolic diseases
medicine.disease
Endocrinology
chemistry
Lipogenesis
Disease Models
RNA
Long Noncoding
Steatosis
Food Science
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Nutrients, Vol 13, Iss 2952, p 2952 (2021), Nutrients, Nutrients; Volume 13; Issue 9; Pages: 2952
- Accession number :
- edsair.doi.dedup.....1c23bb3df9129376224ea1754aeb9d2e