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Up-regulation of interleukin-12 receptor expression in peripheral blood mononuclear cells of patients with HTLV-I-associated myelopathy/tropical spastic paraparesis
- Source :
- Journal of the Neurological Sciences. 196:21-26
- Publication Year :
- 2002
- Publisher :
- Elsevier BV, 2002.
-
Abstract
- We previously reported elevated levels of serum interleukin-12 (IL-12) in association with increased interferon-γ (IFN-γ) levels in patients with human T-lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP). The interaction between IL-12 and IL-12 receptor (IL-12R) plays an important role in differentiation of the T helper type 1 (Th1) phenotype. In this study, we further examined the IL-12/IL-12R axis by investigating the expression of IL-12R and CD40 ligand (CD40L) in peripheral blood mononuclear cells (PBMC) of 18 HAM/TSP patients, and comparing the levels with those in 25 patients with other neurological disorders, including 4 anti-HTLV-I-seropositive carriers as controls. Two-color analysis by flow cytometry revealed a significantly high percentage of IL-12R β1 + cells in CD4 + T lymphocytes in HAM/TSP patients compared to the control. Furthermore, IL-12R β2 mRNA expression in PBMC was detected by reverse-transcriptase polymerase chain reaction in 6 of 18 HAM/TSP patients, but not in any control patients. In contrast, there was no significant difference between the percentage of CD40L + cells in CD4 + T lymphocytes in HAM/TSP and control patients. Our results suggest Th1 immune activation in patients with HAM/TSP, which leads to chronic inflammation in the spinal cord, mediated by dysregulation of the IL-12/IL-12R axis rather than of the CD40/CD40L interaction.
- Subjects :
- Adult
CD4-Positive T-Lymphocytes
Male
Inflammation
Peripheral blood mononuclear cell
Myelopathy
immune system diseases
Interferon
Tropical spastic paraparesis
medicine
Humans
RNA, Messenger
CD40 Antigens
Aged
CD40
biology
business.industry
Receptors, Interleukin-12
virus diseases
Receptors, Interleukin
Middle Aged
Flow Cytometry
medicine.disease
Interleukin-12
Paraparesis, Tropical Spastic
Up-Regulation
Phenotype
Neurology
Interleukin-12 receptor
Immunology
Leukocytes, Mononuclear
biology.protein
Htlv i associated myelopathy
Female
Neurology (clinical)
medicine.symptom
business
medicine.drug
Subjects
Details
- ISSN :
- 0022510X
- Volume :
- 196
- Database :
- OpenAIRE
- Journal :
- Journal of the Neurological Sciences
- Accession number :
- edsair.doi.dedup.....1c4b69c69e8ec43cd1e552e1cd2c48fd
- Full Text :
- https://doi.org/10.1016/s0022-510x(02)00016-3