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Photodynamic Properties of Vital Dyes for Vitreoretinal Surgery
- Source :
- Ophthalmologica. 228:234-238
- Publication Year :
- 2012
- Publisher :
- S. Karger AG, 2012.
-
Abstract
- The purpose of this study was to evaluate photodynamic properties of indocyanine green (ICG), brilliant blue G (BBG) and trypan blue (TB) as currently used vital dyes for chromovitrectomy. Under consideration of intraoperative illumination intensities and dye concentrations, a simulative in vitro investigation was set up. Therefore, standardized dilutions of original ICG, BBG and TB vials were irradiated at a wavelength of 366 nm with an intensity of 14 µW/cm2 between 0 and 48 h. After this, all samples were measured spectroscopically in a 220- to 750-nm bandwidth. Analyzing the vital dyes over the time course, an exponential photolysis was observed for ICG, whereas BBG and TB presented photostable properties. Regarding ICG, 5% of the concentration was degraded to toxic metabolites every 20 min. For this reason, our study provides evidence that intraocular dye concentrations and modern endoillumination systems alone cannot fully prevent ICG photodegradation.
- Subjects :
- Indocyanine Green
Light
Cell Survival
Trypan Blue
General Medicine
Vitreoretinal surgery
Vitreoretinal Surgery
Flow Cytometry
Sensory Systems
Vitreous Body
Ophthalmology
chemistry.chemical_compound
chemistry
Spectrophotometry
Time course
Rosaniline Dyes
Humans
Indicators and Reagents
Trypan blue
Coloring Agents
Photodegradation
Indocyanine green
Brilliant blue G
Nuclear chemistry
Subjects
Details
- ISSN :
- 14230267 and 00303755
- Volume :
- 228
- Database :
- OpenAIRE
- Journal :
- Ophthalmologica
- Accession number :
- edsair.doi.dedup.....1c5be90648c1ad16854efc10544c2a2a
- Full Text :
- https://doi.org/10.1159/000341605