Back to Search
Start Over
Increased FSHD region gene1 expression reduces in vitro cell migration, invasion, and angiogenesis, ex vivo supported by reduced expression in tumors
- Source :
- Bioscience Reports
- Publication Year :
- 2017
- Publisher :
- Portland Press Ltd., 2017.
-
Abstract
- Facioscapulohumeral muscular dystrophy (FSHD) region gene 1 (FRG1) is a candidate gene for FSHD. FRG1 regulates various muscle-related functions, but studies have proposed its role in development and angiogenesis also, where it is involved with tumor-associated molecules. Therefore, we decided to look into its role in tumor progression, tumor angiogenesis, and its impact on cellular properties. Cell proliferation, migration, invasion and in vitro angiogenesis assays were performed to decipher the effect of FRG1 on endothelial and epithelial cell functions. Q-RT PCR was done for human embyonic kidney (HEK293T) cells with altered FRG1 levels to identify associated molecules. Further, immunohistochemistry was done to identify FRG1 expression levels in various cancers and its association with tumor angiogenesis. Subsequently, inference was drawn from Oncomine and Kaplan–Meier plotter analysis, for FRG1 expression in different cancers. Ectopic expression of FRG1 affected cell migration and invasion in both HEK293T and human umbilical vein endothelial cells (HUVECs). In HUVECs, FRG1 overexpression led to reduced angiogenesis in vitro. No effect was observed in cell proliferation in both the cell types. Q-RT PCR data revealed reduction in granulocyte-colony stimulating factor (G-CSF) expression with FRG1 overexpression and increased expression of matrix metalloproteinase 10 (MMP10) with FRG1 knockdown. Immunohistochemistry analysis showed reduced FRG1 levels in tumors which were supported by in silico analysis data. These findings suggest that reduction in FRG1 expression in gastric, colon and oral cavity tumor might have a role in tumor progression, by regulating cell migration and invasiveness. To elucidate a better understanding of molecular signaling involving FRG1 in angiogenesis regulation, further study is required.
- Subjects :
- 0301 basic medicine
cell migration
Angiogenesis
medicine.disease_cause
Biochemistry
angiogenesis
0302 clinical medicine
Cell Movement
Neoplasms
Granulocyte Colony-Stimulating Factor
Research Articles
Gene knockdown
Neovascularization, Pathologic
Microfilament Proteins
Nuclear Proteins
RNA-Binding Proteins
Cell migration
cell invasion
Immunohistochemistry
Gene Expression Regulation, Neoplastic
030220 oncology & carcinogenesis
Gene Knockdown Techniques
Research Article
Cell type
Biophysics
Biology
03 medical and health sciences
Matrix Metalloproteinase 10
oncogenesis
medicine
Human Umbilical Vein Endothelial Cells
Humans
Neoplasm Invasiveness
Molecular Biology
Cell Proliferation
Cell growth
Endothelial Cells
Epithelial Cells
Cell Biology
FRG1
Molecular biology
030104 developmental biology
HEK293 Cells
Tumor progression
Cancer research
Ectopic expression
Carcinogenesis
Subjects
Details
- Language :
- English
- ISSN :
- 15734935 and 01448463
- Volume :
- 37
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Bioscience Reports
- Accession number :
- edsair.doi.dedup.....1c7a7a1f60efa29a6139c4801e971aa6