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Beneficial role of Pistacia lentiscus aqueous extract in experimental colitis: anti-inflammatory and potential therapeutic effects
- Source :
- Inflammopharmacology. 29(4)
- Publication Year :
- 2021
-
Abstract
- Background: Pistacia lentiscus L. (PL) is a flowering plant traditionally used in the treatment of gastrointestinal disorders. The extracts of this plant are endowed with strong pharmacological activities. The aim of our current study was to investigate the anti-inflammatory and potential therapeutic effects of PL leaves aqueous extract (PLAE) against Dextran Sulfate Sodium (DSS)-induced acute colitis. Materials and methods: The therapeutic effect of PLAE was evaluated after orally administration of 3% DSS alone or concomitantly with PLAE (50, 100 or 200 mg/Kg). Mucosal lesions were assessed by macroscopic and histopathological examination. In this context, hemorrhage, diarrhea, weight loss, and disease activity index (DAI) were determined daily throughout the experiment. In the same way, hematoxylin-eosin and Alcian blue staining of colonic mucosal were used to evaluate respectively mucosal damages and mucus production. Furthermore, the levels of nitric oxide (NO), and pro-inflammatory cytokines [tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6)] were measured in plasma, as well as in colonic explants and peritoneal macrophages cultures supernatants.Results: Administration of DSS+PLAE indicated a significant reduction in clinical score of acute colitis DAI compared to DSS alone administration. Interestingly, histological analysis of the mucosa showed that DSS+PLAE-treated groups exhibited almost normal histology evidenced by an intact epithelium structure and less inflammatory cell infiltration in the mucosa. Alcian bleu staining revealed that DSS+PLAE-treated groups displayed almost normal mucus production. Importantly, a significant decrease in pro-inflammatory mediators (NO, IL-6 and TNF-α) levels in dose dependent manner was reported in plasma, and culture supernatants of colonic explants and peritoneal macrophages from DSS+ PLAE-treated mice compared to the DSS group.Conclusion: Our results showed that the systemic and local anti-inflammatory activities of aqueous leaves extract of PL improve the clinical signs of acute colitis. Our data suggest that PLAE has beneficial effects and could constitute a promising approach against acute ulcerative colitis by targeting the deregulated immune response.
- Subjects :
- 0301 basic medicine
medicine.drug_class
Immunology
Anti-Inflammatory Agents
Context (language use)
Pharmacology
Anti-inflammatory
Nitric oxide
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Immune system
Cell Line, Tumor
Medicine
Animals
Pharmacology (medical)
Acute colitis
Cells, Cultured
Cell Proliferation
Mice, Inbred BALB C
Dose-Response Relationship, Drug
business.industry
Plant Extracts
Dextran Sulfate
Water
Histology
Colitis
Mucus
030104 developmental biology
Treatment Outcome
chemistry
Pistacia
Tumor necrosis factor alpha
Female
business
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 15685608
- Volume :
- 29
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Inflammopharmacology
- Accession number :
- edsair.doi.dedup.....1cc482aa7bc72a1e938899712c271f9b