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Erratum to: Pancreatic cancer cell-derived IGFBP-3 contributes to muscle wasting
- Source :
- Journal of Experimental & Clinical Cancer Research : CR
- Publication Year :
- 2016
- Publisher :
- BioMed Central, 2016.
-
Abstract
- Progressive loss of skeletal muscle, termed muscle wasting, is a hallmark of cancer cachexia and contributes to weakness, reduced quality of life, as well as poor response to therapy. Previous studies have indicated that systemic host inflammatory response regarding tumor development results in muscle wasting. However, how tumor directly regulates muscle wasting via tumor-derived secreted proteins is still largely unknown.In this study, we performed bioinformatics analysis in two datasets of pancreatic ductal adenocarcinoma, which causes cancer cachexia and muscle wasting with the highest prevalence, and uncovered that IGFBP3, which encodes IGF-binding protein-3 (IGFBP-3), is dramatically up-regulated in pancreatic tumor samples. We also verified the wasting effect of IGFBP-3 on C2C12 muscle cells with biochemical and genetic assays.IGFBP-3 potently leads to impaired myogenesis and enhanced muscle protein degradation, the major features of muscle wasting, via IGF signaling inhibition. Moreover, conditioned medium from Capan-1 pancreatic cancer cells, which contains abundant IGFBP-3, significantly induces muscle cell wasting. This wasting effect is potently alleviated by IGFBP3 knockdown in Capan-1 cells or IGFBP-3 antibody neutralization. Strikingly, compared to muscle cells, IGF signaling and proliferation rate of Capan-1 cells were rarely affected by IGFBP-3 treatment.Our results demonstrated that pancreatic cancer cells induce muscle wasting via IGFBP-3 production.
- Subjects :
- 0301 basic medicine
Oncology
Cancer Research
medicine.medical_specialty
MEDLINE
Muscle Development
Myoblasts
03 medical and health sciences
Mice
0302 clinical medicine
Internal medicine
Cell Line, Tumor
medicine
Animals
Humans
Wasting
Muscle Weakness
business.industry
Published Erratum
Computational Biology
Up-Regulation
Gene Expression Regulation, Neoplastic
Pancreatic Neoplasms
030104 developmental biology
Insulin-Like Growth Factor Binding Protein 3
Pancreatic cancer cell
030220 oncology & carcinogenesis
Culture Media, Conditioned
Immunology
medicine.symptom
Erratum
business
Carcinoma, Pancreatic Ductal
Subjects
Details
- Language :
- English
- ISSN :
- 17569966 and 03929078
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental & Clinical Cancer Research : CR
- Accession number :
- edsair.doi.dedup.....1ce833a6174e85f3621c24b1fa64dc2b