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Amyotrophic lateral sclerosis (ALS) linked mutation in Ubiquilin 2 affects stress granule assembly via TIA‐1
- Source :
- CNS Neuroscience & Therapeutics
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Aims The ubiquilin‐like protein ubiquilin 2 (UBQLN2) is associated with amyotrophic lateral sclerosis and frontotemporal degeneration (ALS/FTD). The biological function of UBQLN2 has previously been shown to be related to stress granules (SGs). In this study, we aimed to clarify the regulatory relationship between UBQLN2 and SGs. Methods In this study, we transfected UBQLN2‐WT or UBQLN2‐P497H plasmids into cell lines (HEK293T, HeLa), and observed the process of SG dynamics by immunofluorescence. Meanwhile, immunoblot analyses the protein changes of stress granules related components. Results We observed that ubiquilin 2 colocalizes with the SG component proteins G3BP1, TIA‐1, ATXN2, and PABPC1. In cells expressing WT UBQLN2 or P497H mutants, in the early stages of SG formation under oxidative stress, the percentage of cells with SGs and the number of SGs per cell decreased to varying degrees. Between WT and mutant, there was no significant difference in eIF2α activity after stress treatment. Interestingly, the UBQLN2 P497H mutant downregulates the level of TIA‐1. In addition, the overexpression of the UBQLN2 P497H mutant inhibited the phosphorylation of 4E‐BP1 and affected the nucleoplasmic distribution of TDP‐43. Conclusions Ubiquilin 2 colocalizes with the SG component proteins G3BP1, TIA‐1, ATXN2, and PABPC1. It participates in regulating SG dynamics. And UBQLN2 mutation affects the assembly of stress granules by regulating TIA‐1. In addition, the overexpression of the UBQLN2 P497H mutant inhibited the phosphorylation of 4E‐BP1 and affected the nuclear and cytoplasmic distribution of TDP‐43. These provide new insights into the role of UBQLN2 in oxidative stress and the pathogenesis of ALS.<br />Stresses activate eIF2‐Á kinases that phosphorylate eIF2‐Á, deplete the ternary complex, and promote the assembly of a noncanonical Pre‐initation complex£¨PIC£©; these interfaces recruit RNA‐BPs such as TIA‐1, increasing the local concentration of these proteins to promote LLPS and assembly of SG seeds. However, UBQLN2 P497H could repress the level of TIA‐1, thereby inhibiting stress granule assembly.
- Subjects :
- amyotrophic lateral sclerosis
stress granules
Mutant
Autophagy-Related Proteins
Protein aggregation
protein aggregation
UBQLN2
Stress granule
Physiology (medical)
TIA‐1
Humans
Pharmacology (medical)
Stress granule assembly
Poly-ADP-Ribose Binding Proteins
Adaptor Proteins, Signal Transducing
Pharmacology
biology
Chemistry
HEK 293 cells
DNA Helicases
Original Articles
Transfection
T-Cell Intracellular Antigen-1
Cell biology
Psychiatry and Mental health
HEK293 Cells
RNA Recognition Motif Proteins
Frontotemporal Dementia
Mutation
biology.protein
Phosphorylation
Original Article
RNA Helicases
Subjects
Details
- ISSN :
- 17555949 and 17555930
- Volume :
- 28
- Database :
- OpenAIRE
- Journal :
- CNS Neuroscience & Therapeutics
- Accession number :
- edsair.doi.dedup.....1d4d29dac1b77ae828bcb16637dd7c57