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Programming Nanopore Ion Flow for Encoded Multiplex MicroRNA Detection
- Source :
- ACS Nano
- Publication Year :
- 2014
- Publisher :
- American Chemical Society, 2014.
-
Abstract
- Many efforts are being made in translating the nanopore into an ultrasensitive single-molecule platform for various genetic and epigenetic detections. However, compared with current approaches including PCR, the low throughput limits the nanopore applications in biological research and clinical settings, which usually requires simultaneous detection of multiple biomarkers for accurate disease diagnostics. Herein we report a barcode probe approach for multiple nucleic acid detection in one nanopore. Instead of directly identifying different targets in a nanopore, we designed a series of barcode probes to encode different targets. When the probe is bound with the target, the barcode group polyethylene glycol attached on the probe through click chemistry can specifically modulate nanopore ion flow. The resulting signature serves as a marker for the encoded target. Therefore counting different signatures in a current recording allows simultaneous analysis of multiple targets in one nanopore. The principle of this approach was verified by using a panel of cancer-derived microRNAs as the target, a type of biomarker for cancer detection.
- Subjects :
- Ion flow
General Physics and Astronomy
Nanotechnology
02 engineering and technology
Computational biology
Biology
010402 general chemistry
Barcode
ENCODE
01 natural sciences
Article
law.invention
Nanopores
law
General Materials Science
Multiplex
nanopore
microRNA
multiplex detection
Hybridization probe
General Engineering
barcode
Nucleic Acid Hybridization
021001 nanoscience & nanotechnology
3. Good health
0104 chemical sciences
Nanopore
MicroRNAs
click chemistry
Nanopore sequencing
0210 nano-technology
DNA Probes
Biomarkers
Nucleic acid detection
Subjects
Details
- Language :
- English
- ISSN :
- 1936086X and 19360851
- Volume :
- 8
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- ACS Nano
- Accession number :
- edsair.doi.dedup.....1d80937bc1512b76fa09a9744d36126a