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Clonal Dissemination of OXA-370-Producing Klebsiella pneumoniae in Rio de Janeiro, Brazil
- Source :
- Antimicrobial Agents and Chemotherapy. 59:4453-4456
- Publication Year :
- 2015
- Publisher :
- American Society for Microbiology, 2015.
-
Abstract
- Enzymes of the OXA-48 family have become some of the most important beta-lactamases in the world. A new OXA-48 variant (OXA-370) was first described for an Enterobacter hormaechei strain isolated in Rio Grande do Sul (southern region of Brazil) in 2013. Here we report detection of the bla OXA-370 gene in 24 isolates belonging to three Enterobacteriaceae species (22 Klebsiella pneumoniae isolates, 1 Enterobacter cloacae isolate, and 1 Enterobacter aerogenes isolate) collected from five hospitals in Rio de Janeiro, Brazil, in 2013 and 2014. The isolates showed a multidrug resistance profile, and 12.5% were resistant to polymyxin B. Besides bla OXA-370 , no other carbapenemase genes were observed by PCR, whereas bla OXA-1 was found in all isolates and 22 isolates (91.6%) possessed bla CTX-M-15 . Molecular typing of the K. pneumoniae isolates by pulsed-field gel electrophoresis (PFGE) showed the presence of two clonal groups, i.e., KpA (21 isolates) and KpB (1 isolate). KpA was characterized as sequence type 16 (ST16) and KpB as ST1041 by multilocus sequence typing (MLST). ST16 has been observed for KPC-producing K. pneumoniae in Rio de Janeiro. Plasmid analysis performed with six representative OXA-370-producing isolates showed plasmids harboring the bla OXA-370 gene in all strains, ranging from 25 kb to 150 kb. This study suggests that there is an urgent need to investigate the presence of OXA-370 and dissemination of the K. pneumoniae ST16 clone carrying this gene in Brazil.
- Subjects :
- Pharmacology
biology
Klebsiella pneumoniae
biology.organism_classification
Enterobacter aerogenes
Enterobacteriaceae
Virology
beta-Lactamases
Bacterial Typing Techniques
Klebsiella Infections
Epidemiology and Surveillance
Microbiology
Multiple drug resistance
Infectious Diseases
Plasmid
Bacterial Proteins
Drug Resistance, Multiple, Bacterial
Pulsed-field gel electrophoresis
Multilocus sequence typing
Pharmacology (medical)
Enterobacter cloacae
Brazil
Plasmids
Subjects
Details
- ISSN :
- 10986596 and 00664804
- Volume :
- 59
- Database :
- OpenAIRE
- Journal :
- Antimicrobial Agents and Chemotherapy
- Accession number :
- edsair.doi.dedup.....1d8f2b28ec77263296f0d3e4a04bbba9