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Analysis of infantile fibrosarcoma reveals extensive T-cell responses within tumors: Implications for immunotherapy

Authors :
Chao Xin
Jingyan Tang
Ci Pan
Chenjie Xie
Hua Zhu
Min Shi
Jing Wang
Siqi Huang
Minzhi Yin
Song Gu
Xue-Feng Bai
Jing Ma
Jianmin Zhu
Min Xu
Source :
Pediatric Blood & Cancer. 65:e26813
Publication Year :
2017
Publisher :
Wiley, 2017.

Abstract

Background Infantile fibrosarcoma (IFS) is a rare pediatric malignancy with relatively good prognosis, but the risk of progression or recurrence after therapy exists. To understand the immune microenvironment of IFS and determine if immunotherapy is a potential treatment, we analyzed T-cell responses in IFS tumors. Procedure IFS tumors were analyzed by immunohistochemistry and multicolor flow cytometry to characterize immune cell infiltration and function. Tumor infiltrating lymphocytes (TILs) were expanded in vitro and evaluated for recognition of autologous tumor cells. Real-time PCR was applied to evaluate tumor expression of chemokines/cytokines and tumor antigens. Results Significant infiltration of both CD4+ and CD8+ T cells was found in seven of 10 IFS but rarely found in age- and sex-matched rhabdomyosarcoma tumors. The TILs from recurrent IFS tumors expressed high levels of costimulatory molecules such as CD28, 4-1BB, and OX40, but little or no coinhibitory molecules such as PD-1 and CTLA4, Tim3, Lag3, and CD39. Upon activation, large portions of TILs produced IFN-γ and TNF-α. Eighteen out of 40 T cell lines generated from surgically removed tumors could recognize autologous tumor cells. Moreover, we found that IFS tumors expressed high levels of T-cell chemokines such as CXCL10 and CXCL16, and also classic tumor antigens such as CTAG2, GAGE, and NY-ESO-1, whose expression could be further enhanced by treatment with epigenetic modulator decitabine. Conclusions IFS tumors are highly immunogenic and expansion of TILs followed by adoptive cell transfer could be a potential immunotherapy for IFS patients undergoing tumor recurrence.

Details

ISSN :
15455009
Volume :
65
Database :
OpenAIRE
Journal :
Pediatric Blood & Cancer
Accession number :
edsair.doi.dedup.....1d9d8f2c60e818908fcc6e87ada1a68f
Full Text :
https://doi.org/10.1002/pbc.26813