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Detection of Placental Proteomes at Different Uterine Positions in Large White and Meishan Gilts on Gestational Day 90
- Source :
- PLoS ONE, PLoS ONE, Vol 11, Iss 12, p e0167799 (2016)
- Publication Year :
- 2016
- Publisher :
- Public Library of Science, 2016.
-
Abstract
- Within-litter uniformity in pigs is a major factor affecting piglet survival and growth performance. We know that Meishan (MS) gilts have higher piglet survival rate than Large White (LW) gilts because their foetal weight is less varied. To understand the molecular basis for placental nutritional transport during the late stages of gestation in LW and MS, we employed the isobaric tags for relative and absolute quantification (iTRAQ) method to investigate alterations in the placental proteomes of LW and MS gilts on gestational day 90. Investigation of foetal weight at different uterine positions revealed that the foetal and placental weights as well as the foetal concentration of glucose were significantly higher in LW gilts positioned towards the utero-tubal junction than in those positioned toward the cervix; however, no such differences were observed in MS gilts, and MS gilts had a greater uniformity in foetal weight on day 90 of gestation. Comparisons of the proteomes between placentas positioned toward the cervix and those positioned toward the utero-tubal junction identified 38 differentially expressed proteins in the two breeds. These proteins play a central role in nutrient transport and metabolism, as well as in transcriptional and translational regulation. Of particular interest is the finding that the placentas of LW gilts showed 14 differential expression of proteins mainly related to lipid transport and energy metabolism (including solute carrier family 27, mitochondrial trifunctional protein, and NADH dehydrogenase [ubiquinone] flavoprotein 2), but only 2 proteins in MS gilts. In contrast, the differentially expressed proteins in MS gilts were primarily involved in transcriptional and translational regulation (such as ribosome-sec61 and 40S ribosomal protein S23), with a few related to glucose and coenzyme transport and metabolism (including glucose transport protein and ferrochelatase). Our results revealed that placental lipid and energy metabolism might play a crucial role in the regulation of foetal weight, based on uterine position in two distinct pig breeds. These findings provide a deeper understanding of placental efficiency that can be utilized to provide a new method to enhance the efficiency of livestock production.
- Subjects :
- 0301 basic medicine
Blood Glucose
Embryology
Litter Size
Proteome
Swine
Placenta
Protein Expression
Cell Membranes
lcsh:Medicine
Mitochondrial trifunctional protein
Breeding
Biochemistry
Cervix
0302 clinical medicine
Glucose Metabolism
Pregnancy
Translational regulation
Medicine and Health Sciences
Amino Acids
Insulin-Like Growth Factor I
lcsh:Science
Protein Metabolism
030219 obstetrics & reproductive medicine
Multidisciplinary
biology
Lipids
Fetal Weight
Gestation
Carbohydrate Metabolism
Animal Nutritional Physiological Phenomena
Female
Coenzyme transport
Anatomy
Cellular Structures and Organelles
Genital Anatomy
Research Article
Carbohydrate metabolism
Research and Analysis Methods
Andrology
03 medical and health sciences
Gene Expression and Vector Techniques
Animals
Molecular Biology Techniques
Molecular Biology
Molecular Biology Assays and Analysis Techniques
lcsh:R
Uterus
Reproductive System
Biology and Life Sciences
Membrane Proteins
Lipid metabolism
Biological Transport
Metabolism
Cell Biology
Lipid Metabolism
Solute carrier family
030104 developmental biology
Gene Expression Regulation
biology.protein
lcsh:Q
Energy Metabolism
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 11
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....1db32684720394a2db71d27c1d3c7b26