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Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes
- Source :
- Acta histochemica. 121(5)
- Publication Year :
- 2018
-
Abstract
- This study investigated the effects of a CoCl2-simulated hypoxic environment on the muscle fiber switching signaling pathways calcineurin A/nuclear factor of activated T cells cytoplasmic 1 (CnA/NFATc1) and myostatin. In this study, C2C12 muscle cells were cultured in vitro under CoCl2-simulated chemical hypoxic conditions, the expression levels of CnA and myostatin were detected through qRT-PCR and Western blot analyses, and a positioning study of NFATc1 was carried out by immunofluorescence labeling. Results showed that CoCl2 treatment significantly increased the expression levels of CnA and myostatin. Moreover, the position of NFATc1 expression changed; actually, its expression in the nucleus considerably increased. Furthermore, CoCl2-induced hypoxia inhibited the differentiation of C2C12 cells and reduced the expression levels of many slow- and fast-twitch muscles marker genes, but immunofluorescence staining results showed that the proportion of MyHC I type muscle fiber increased after CoCl2 treatment. The hypoxic environment simulated by CoCl2 can activate the signaling pathways CnA/NFATc1 and myostatin and increases the proportion of MyHC I type muscle fibers.
- Subjects :
- 0301 basic medicine
Histology
Blotting, Western
Myostatin
Immunofluorescence
Real-Time Polymerase Chain Reaction
03 medical and health sciences
Mice
0302 clinical medicine
Western blot
medicine
Myocyte
Animals
Muscle, Skeletal
Cells, Cultured
biology
medicine.diagnostic_test
NFATC Transcription Factors
Chemistry
Calcineurin
Cell Biology
General Medicine
Cobalt
Hypoxia (medical)
musculoskeletal system
In vitro
Cell Hypoxia
Cell biology
030104 developmental biology
030220 oncology & carcinogenesis
biology.protein
Signal transduction
medicine.symptom
Transcriptome
C2C12
Signal Transduction
Subjects
Details
- ISSN :
- 16180372
- Volume :
- 121
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Acta histochemica
- Accession number :
- edsair.doi.dedup.....1db362d4e1aff2a1ce3e19869bb563a9