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Copy Number and Prevalence of Porcine Endogenous Retroviruses (PERVs) in German Wild Boars
- Source :
- Viruses, 12(4):419, Viruses, Vol 12, Iss 419, p 419 (2020), Viruses, Volume 12, Issue 4
- Publication Year :
- 2020
- Publisher :
- Robert Koch-Institut, 2020.
-
Abstract
- Porcine endogenous retroviruses (PERVs) are integrated in the genome of pigs and are transmitted like cellular genes from parents to the offspring. Whereas PERV-A and PERV-B are present in all pigs, PERV-C was found to be in many, but not all pigs. When PERV-C is present, recombination with PERV-A may happen and the PERV-A/C recombinants are characterized by a high replication rate. Until now, nothing has been known about the copy number of PERVs in wild boars and little is known about the prevalence of the phylogenetically youngest PERV-C in ancient wild boars. Here we investigated for the first time the copy number of PERVs in different populations of wild boars in and around Berlin using droplet digital PCR. Copy numbers between 3 and 69 per genome have been measured. A lower number but a higher variability was found compared to domestic pigs, including minipigs reported earlier (Fiebig et al., Xenotransplantation, 2018). The wild boar populations differed genetically and had been isolated during the existence of the Berlin wall. Despite this, the variations in copy number were larger in a single population compared to the differences between the populations. PERV-C was found in all 92 analyzed animals. Differences in the copy number of PERV in different organs of a single wild boar indicate that PERVs are also active in wild boars, replicating and infecting new cells as has been shown in domestic pigs.
- Subjects :
- 0301 basic medicine
endocrine system
DNA Copy Number Variations
Swine
Offspring
Xenotransplantation
medicine.medical_treatment
Sus scrofa
030106 microbiology
Population
lcsh:QR1-502
Endogenous retrovirus
Genome
Article
lcsh:Microbiology
03 medical and health sciences
Proviruses
Wild boar
Germany
Virology
biology.animal
copy number
Prevalence
medicine
Animals
Digital polymerase chain reaction
ddc:610
education
Gene
Genetics
education.field_of_study
biology
porcine endogenous retroviruses (PERV)
Endogenous Retroviruses
030104 developmental biology
Infectious Diseases
wild boars
PERV-C
610 Medizin und Gesundheit
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Viruses, 12(4):419, Viruses, Vol 12, Iss 419, p 419 (2020), Viruses, Volume 12, Issue 4
- Accession number :
- edsair.doi.dedup.....1db775214f150c796c13515847a8658b