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Assessment of the Biological Activity and Phenolic Composition of Ethanol Extracts of Pomegranate (Punica granatum L.) Peels
- Source :
- Molecules, Volume 25, Issue 24, Molecules, Vol 25, Iss 5916, p 5916 (2020)
- Publication Year :
- 2020
-
Abstract
- Pomegranate (Punica granatum L.) is a rich source of constituents with confirmed strong biological activities. However, pomegranate peel, which encompasses approximately 30&ndash<br />40% of its weight, is treated as a biological waste. The aim of this paper was to evaluate the potential of pomegranate peel extracts and to propose its functional properties that can be used for development of functional products. Eight ethanol extracts of pomegranate peels (PPEs) were characterized by use of direct infusion quadrupole-time of flight (Q-TOF), and afterwards tested on their antioxidant, antibacterial and antiproliferative activities. Mass spectrometry analysis revealed that the most prevalent compounds in pomegranate peels were punicalagin, granatin and their derivatives. Analysed extracts had high total phenolic contents that ranged from 5766.44 to 10599.43 mg GAE/100 g, and strong antioxidant activity (7551.31&ndash<br />7875.42 and 100.25&ndash<br />176.60 &mu<br />mol TE/100 g for DPPH and FRAP assays, respectively). The results of biological activity assays showed that all PPEs possessed antibacterial activity, and that S. aureus was the most sensitive specie with minimum inhibitory concentration and minimum bactericidal concentrations ranging from 0.8 to 6.4 mg/mL. Additionally, the analysis of antiproliferative activity revealed high potency of PPEs, as the IC50 values ranged from 0.132 mg/mL to 0.396 mg/mL. Multivariate analysis pointed out the most discriminative metabolites for antioxidant or antiproliferative activity. Overall, the pomegranate peel confirmed to be a highly valuable source of bioactive compounds that could be used to improve the food functional characteristics.
- Subjects :
- Antioxidant
DPPH
medicine.medical_treatment
Pharmaceutical Science
antioxidant activity
01 natural sciences
Antioxidants
Pomegranate
Analytical Chemistry
pomegranate peel
chemistry.chemical_compound
BIOMEDICINE AND HEALTHCARE. Clinical Medical Sciences. Medical Microbiology
antibacterial activity
Drug Discovery
Food science
Principal Component Analysis
biology
Biological activity
04 agricultural and veterinary sciences
040401 food science
Hydrolyzable Tannins
Anti-Bacterial Agents
Chemistry (miscellaneous)
Punica
Molecular Medicine
Antibacterial activity
antiproliferative activity
Cell Survival
Microbial Sensitivity Tests
Gram-Positive Bacteria
Article
lcsh:QD241-441
Inhibitory Concentration 50
Minimum inhibitory concentration
0404 agricultural biotechnology
Phenols
Picrates
lcsh:Organic chemistry
Cell Line, Tumor
Gram-Negative Bacteria
medicine
Humans
Potency
Physical and Theoretical Chemistry
Punicalagin
Waste Products
Microbial Viability
Plant Extracts
Biphenyl Compounds
010401 analytical chemistry
Organic Chemistry
biology.organism_classification
phytochemicals
Antineoplastic Agents, Phytogenic
0104 chemical sciences
chemistry
Fruit
BIOTEHNIČKE ZNANOSTI. Prehrambena tehnologija
BIOTECHNICAL SCIENCES. Food Technology
BIOMEDICINA I ZDRAVSTVO. Kliničke medicinske znanosti. Medicinska mikrobiologija
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules, Volume 25, Issue 24, Molecules, Vol 25, Iss 5916, p 5916 (2020)
- Accession number :
- edsair.doi.dedup.....1dcd2c26ac4429483aa3c19a6b229c23