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Protective effects of hypercapnic acidosis on Ischemia–reperfusion-induced retinal injury
- Source :
- PLoS ONE, PLoS ONE, Vol 14, Iss 1, p e0211185 (2019)
- Publication Year :
- 2019
- Publisher :
- Public Library of Science, 2019.
-
Abstract
- Ischemia-reperfusion (I/R) injury is associated with numerous retinal diseases, such as diabetic retinopathy, acute glaucoma, and other vascular retinopathies. Hypercapnic acidosis (HCA) has a protective effect on lung, myocardial, and central nervous system ischemic injury models. However, no study has evaluated its protective effects in an experimental retinal I/R injury model. In this study, retinal I/R injury was induced in Sprague Dawley rats by elevating the intraocular pressure to 110 mmHg for 60 minutes. HCA was induced before and after the injury. After 24 hours, the terminal dUTP nick end labeling assay was performed. Moreover, the ratios of cleaved caspase-3/total caspase-3, phosphorylated IκB/IκB, and phosphorylated p38 were measured through Western blotting. After 7 days, the rats' aqueous humor was analyzed. In addition, electroretinography and retinal thickness measurement were performed in the rats. Moreover, the retinal neural cell line RGC-5 was exposed to 500 μM H2O2 for 24 hours to induce a sustained oxidative stress in vitro. The effects of HCA were evaluated by comparing oxidative stress, MAPK signals, NF-κB signals, survival rates, and apoptosis rates in the RGC-5 cells before and after H2O2 exposure. We further investigated whether the potent I/R-protective heat shock protein (HSP) 32 contribute to protective effects of HCA. Our results indicated that HCA has protective effects against retinal I/R injury both in vivo and in vitro, at multiple levels, including antiapoptotic, anti-inflammatory, antioxidative, and functional retinal cell protection. Further research clarifying the role of HCA in retinal I/R injury prevention and treatment is warranted.
- Subjects :
- MAPK/ERK pathway
Male
Cell signaling
Apoptosis
Pharmacology
Signal transduction
medicine.disease_cause
Pathology and Laboratory Medicine
Hypercapnia
Rats, Sprague-Dawley
chemistry.chemical_compound
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
Immune Response
Neurons
Multidisciplinary
medicine.diagnostic_test
Cell Death
Signaling cascades
Cell Processes
Reperfusion Injury
Medicine
Anatomy
Cellular Types
Acidosis
Research Article
MAPK signaling cascades
MAP Kinase Signaling System
p38 mitogen-activated protein kinases
Science
Ocular Anatomy
Immunology
Ischemia
Retina
Cell Line
03 medical and health sciences
Signs and Symptoms
Retinal Diseases
In vivo
Diagnostic Medicine
Ocular System
medicine
Animals
Eye Proteins
Inflammation
business.industry
Biology and Life Sciences
Retinal
Cell Biology
medicine.disease
Rats
Oxidative Stress
chemistry
Cellular Neuroscience
030221 ophthalmology & optometry
Eyes
business
Head
030217 neurology & neurosurgery
Oxidative stress
Electroretinography
Neuroscience
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....1df602c6e3918f2b91b650d0a1aeb950