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An Immunogenic Peptide, T2 Induces Interstitial Cystitis/Painful Bladder Syndrome: an Autoimmune Mouse Model for Interstitial Cystitis/Painful Bladder Syndrome
- Source :
- Inflammation. 40(6)
- Publication Year :
- 2017
-
Abstract
- The exact pathophysiology of interstitial cystitis/painful bladder syndrome is unknown; however, autoimmunity is a valid theory. We developed an autoimmune chronic cystitis model by administration of the medium dose of immunogenic peptide T2. Sixty female C57BL/6 mice were divided into six groups. The control group was not treated with any reagent. CFA group was injected with CFA + normal saline, homogenate group with bladder homogenate + CFA, low-dose group with low dose of T2 peptide + CFA, medium dose group with the medium dose of T2 peptide + CFA, and high-dose group with the high dose of T2 peptide + CFA. Micturition habits, withdrawal frequencies of mice, and bladders weight were measured for each group. Hematoxylin and eosin staining and toluidine blue staining were used to investigate bladder inflammation and mast cells accumulation, respectively. T cells infiltration in the bladder tissues and serum TNF-α level were measured by using immunohistochemistry and ELISA, respectively. Mice immunized with the medium dose of T2 peptide (0.225 mg/ml) were extremely sensitive to the applied force, showed greater urine frequencies, and higher bladder weights. Histologic examination revealed severe edema and inflammation in bladder tissues of medium-dose group. Extensive infiltration of T cells in bladder tissues, elevated TNF-α, and increased mast cells accumulation were observed in medium-dose group as compared to that in other groups. EAC mice model established by injecting the medium dose of T2 (0.225 mg/ml) mimics all the symptoms and pathophysiologic characteristics of IC/PBS. We believe that this model can help us to investigate the pathogenesis of IC/PBS.
- Subjects :
- Pathology
medicine.medical_specialty
media_common.quotation_subject
Immunology
Urinary Bladder
030232 urology & nephrology
H&E stain
Cystitis, Interstitial
Inflammation
Autoimmunity
Urination
03 medical and health sciences
Mice
0302 clinical medicine
medicine
Immunology and Allergy
Animals
media_common
business.industry
Interstitial cystitis
Chronic Cystitis
Organ Size
medicine.disease
Urination Disorders
Pathophysiology
Disease Models, Animal
030220 oncology & carcinogenesis
Tumor necrosis factor alpha
Female
medicine.symptom
business
Peptides
Infiltration (medical)
Subjects
Details
- ISSN :
- 15732576
- Volume :
- 40
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Inflammation
- Accession number :
- edsair.doi.dedup.....1e04c762b8c3a6fb1a730763dc20e708