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In Vitro and In Silico Analysis of miR-125a with rs12976445 Polymorphism in Breast Cancer Patients
- Source :
- Applied Sciences, Vol 10, Iss 7275, p 7275 (2020), Applied Sciences, Volume 10, Issue 20
- Publication Year :
- 2020
- Publisher :
- MDPI AG, 2020.
-
Abstract
- Background: Breast cancer affects over 2 million women yearly. Its early detection allows for successful treatment, which motivates to research factors that enable an accurate diagnosis. miR-125a is one of them, correlating with different types of cancer. For example, the miR-125a level decreases in breast cancer tissues<br />polymorphisms in the miR-125a encoding gene are related to prostate cancer and the risk of radiotherapy-induced pneumonitis. Methods: In this work, we investigated two variants of rs12976445 polymorphism in the context of breast cancer. We analyzed the data of 175 blood samples from breast cancer patients and compared them with the control data from 129 control samples. Results: We observed the tendency that in breast cancer cases TT genotype appeared slightly more frequent over CC and CT genotypes (statistically nonsignificant). The TT genotype appeared also to be more frequent among human epidermal growth factor receptor 2 (HER2) positive patients, compared to HER2 negative. In silico modelling showed that the presence of uridine (U) diminished the probability of pri-miR-125a binding to NOVA1 and HNRNPK proteins. We demonstrated that U and C-variants could promote different RNA folding patterns and provoke alternative protein binding. Conclusions: U-variant may imply a lower miR-125a expression in breast cancer.
- Subjects :
- 0301 basic medicine
Oncology
medicine.medical_specialty
In silico
lcsh:Technology
polymorphism
lcsh:Chemistry
03 medical and health sciences
Prostate cancer
0302 clinical medicine
Breast cancer
breast cancer
Internal medicine
Genotype
microRNA
medicine
General Materials Science
RNA folding
Instrumentation
Gene
lcsh:QH301-705.5
Pneumonitis
Fluid Flow and Transfer Processes
business.industry
lcsh:T
Process Chemistry and Technology
General Engineering
medicine.disease
RNA protein binding
In vitro
lcsh:QC1-999
Computer Science Applications
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
lcsh:TA1-2040
030220 oncology & carcinogenesis
business
lcsh:Engineering (General). Civil engineering (General)
lcsh:Physics
Subjects
Details
- Language :
- English
- ISSN :
- 20763417
- Volume :
- 10
- Issue :
- 7275
- Database :
- OpenAIRE
- Journal :
- Applied Sciences
- Accession number :
- edsair.doi.dedup.....1e0b62091030d7853eff6c030d1ad8b6