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Chiral Os(II) Polypyridyl Complexes as Enantioselective Nuclear DNA Imaging Agents Especially Suitable for Correlative High-Resolution Light and Electron Microscopy Studies
- Source :
- ACS applied materialsinterfaces. 12(3)
- Publication Year :
- 2020
-
Abstract
- The high-resolution technique transmission electron microscopy (TEM), with OsO4 as the traditional fixative, is an essential tool for cell biology and medicine. Although OsO4 has been extensively used, it is far from perfect because of its high volatility and toxicity. Os(II) polypyridyl complexes like [Os(phen)2(dppz)]2+ (phen = 1,10-phenanthroline; dppz = dipyridophenazine) are not only the well-known molecular DNA “light-switches” but also the potential ideal candidates for TEM studies. Here, we report that the cell-impermeable cationic [Os(phen)2(dppz)]2+ can be preferentially delivered into the live-cell nucleus through ion-pairing with chlorophenolate counter-anions, where it functions as an unparalleled enantioselective nuclear DNA imaging reagent especially suitable for correlative light and electron microscopy (CLEM) studies in both living and fixed cells, which can clearly visualize chromosome aggregation and decondensation during mitosis simultaneously. We propose that the chiral Os(II) polypyr...
- Subjects :
- Materials science
Osmium Tetroxide
Mitosis
02 engineering and technology
010402 general chemistry
01 natural sciences
law.invention
chemistry.chemical_compound
Microscopy, Electron, Transmission
law
Cell Line, Tumor
Animals
Humans
General Materials Science
Cell Nucleus
Microscopy
Enantioselective synthesis
Cationic polymerization
Stereoisomerism
DNA
021001 nanoscience & nanotechnology
Combinatorial chemistry
0104 chemical sciences
Nuclear DNA
chemistry
Transmission electron microscopy
Reagent
Electron microscope
0210 nano-technology
Phenanthrolines
Subjects
Details
- ISSN :
- 19448252
- Volume :
- 12
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- ACS applied materialsinterfaces
- Accession number :
- edsair.doi.dedup.....1e0e5f2853ab811e2f1805843b39c92d