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Distribution of adipocyte-derived leucine aminopeptidase (A-LAP)/ER-aminopeptidase (ERAP)-1 in human uterine endometrium
- Source :
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 52(9)
- Publication Year :
- 2004
-
Abstract
- Adipocyte-derived leucine aminopeptidase (A-LAP, endoplasmic reticulum aminopeptidase ERAP1) is specialized to produce peptides presented on the class I major histocompatibility complex (MHC) by trimming epitopes to eight or nine residues, in addition to its enzymatic activity to degrade angiotensin II. Previously we identified placental leucine aminopeptidase (P-LAP), another member of the oxytocinase subfamily of aminopeptidases, in human uterine endometrial epithelial cells. Here we analyzed the distribution of A-LAP in human cyclic endometrium. Western blotting analysis showed that A-LAP was present in the endometrial tissue throughout the menstrual cycle. Immunohistochemical (IHC) analysis of A-LAP showed a similar distribution to that of P-LAP. A-LAP was localized predominantly in the endometrial glands and the luminal surface epithelium. However, the intracellular localization change that accompanied apocrine secretion, which was observed in P-LAP, was not shown in A-LAP. Subcellular localization of A-LAP, demonstrated by immunofluorescence, was ER in the cultured glandular epithelial cells. Our results indicate that A-LAP may fit the endometrial localization as an antigen-presenting ER aminopeptidase. Further understanding of the function(s) of A-LAP in the endometrium appear likely to yield insights into the cyclic changes during the normal endometrial cycle.
- Subjects :
- 0301 basic medicine
Adult
Histology
Endometrial Cycle
Biology
Endometrium
Aminopeptidase
Aminopeptidases
Minor Histocompatibility Antigens
03 medical and health sciences
medicine
Humans
Menstrual Cycle
030102 biochemistry & molecular biology
Endoplasmic reticulum
digestive, oral, and skin physiology
Apocrine
Epithelial Cells
Middle Aged
equipment and supplies
Subcellular localization
Molecular biology
Angiotensin II
Immunohistochemistry
Epithelium
030104 developmental biology
medicine.anatomical_structure
Biochemistry
Female
Anatomy
human activities
Subjects
Details
- ISSN :
- 00221554
- Volume :
- 52
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
- Accession number :
- edsair.doi.dedup.....1e528f1d0fa9b50a53ddf0b0f2b02829