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Intrachanges as part of complex chromosome-type exchange aberrations
- Source :
- Mutation research-Fundamental and molecular mechanisms of mutagenesis, 504(1-2), 47-55. ELSEVIER SCIENCE BV
- Publication Year :
- 2002
- Publisher :
- Elsevier BV, 2002.
-
Abstract
- The chromosome-type exchange aberrations induced by ionizing radiation during the G(0)/G(1) phase of the cell cycle are believed to be the result of illegitimate rejoining of chromosome breaks. From numerous studies using chromosome painting, it has emerged that even after a moderate dose of radiation, a substantial fraction of these exchanges is complex. Most of them are derived from the free interaction between the ends of three or more breaks. Other studies have demonstrated that chromosomes occupy distinct territories in the interphase nucleus. Since breaks that are in close proximity have an enhanced interaction probability, it seems likely that after ionizing radiation many of the interacting breaks will be present within one chromosome or chromosome arm. Unfortunately, the majority of these intrachanges remain undetected, even when sophisticated molecular cytogenetic detection methods (i.e. mFISH) are applied to paint all chromosome pairs in distinct colors. In the present paper, we evaluate the limitations of full-color painting for the detection of complex exchanges and the correct interpretations of break interactions. (C) 2002 Elsevier Science B.V. All rights reserved.
- Subjects :
- Interphase nucleus
Health, Toxicology and Mutagenesis
proximity effects
Biology
Chromosome Painting
Ionizing radiation
Chromosome Breaks
FISH
Genetics
Humans
Lymphocytes
COMBINATION
TELOMERIC PNA PROBE
Molecular Biology
Chromosome Aberrations
Chromosome type
DNA PROBES
Chromosome
Chromosome Breakage
Dose-Response Relationship, Radiation
IN-SITU HYBRIDIZATION
complex aberrations
HUMAN-LYMPHOCYTES
Chromosome Arm
RADIATION
Chromosome painting
INCOMPLETE EXCHANGES
ionizing radiation
intrachanges
Moderate-Dose
Subjects
Details
- ISSN :
- 00275107
- Volume :
- 504
- Database :
- OpenAIRE
- Journal :
- Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
- Accession number :
- edsair.doi.dedup.....1e7bcfb27d3fd2bd1c5797c91868995e