Back to Search
Start Over
Upregulation of RAGE at the blood-brain barrier in streptozotocin-induced diabetic mice
- Source :
- Synapse (New York, N.Y.). 63(8)
- Publication Year :
- 2009
-
Abstract
- Deposition of amyloid-beta peptide (Abeta) in the brain of diabetes is poorly understood. The receptor for advanced glycation end products (RAGE) at the blood-brain barrier (BBB) is critical for regulation of Abeta homeostasis in the brain. In this studies, we used streptozotocin-induced diabetic mice to observe the expression of RAGE at the BBB by Western blot and immunocytochemical analysis, and the in vivo blood-to-brain influx transport of (125)I-Abeta(1-) (40) using the permeability surface area product (PS) and brain capillary uptake. In the diabetic mice with hyperglycemia (>16.0 mmol/L) at 6 weeks, RAGE expression at the BBB was significantly upregulated, no significant changes of RAGE levels were found at 1 and 3 weeks after diabetes induction. The data of PS and brain capillary uptake for Abeta showed significant RAGE-dependent transport of Abeta across the BBB and substantial RAGE-dependent brain capillary uptake at 6 weeks after diabetes induction. We conclude that the upregulation of RAGE at the BBB contributes to cerebral Abeta deposition in the diabetes.
- Subjects :
- Blood Glucose
Male
medicine.medical_specialty
Time Factors
endocrine system diseases
Receptor for Advanced Glycation End Products
Blood–brain barrier
RAGE (receptor)
Diabetes Mellitus, Experimental
Cellular and Molecular Neuroscience
Mice
Downregulation and upregulation
Isotopes
Glycation
Diabetes mellitus
Internal medicine
mental disorders
medicine
Animals
Receptors, Immunologic
Receptor
Glycated Hemoglobin
Amyloid beta-Peptides
business.industry
nutritional and metabolic diseases
Brain
medicine.disease
Streptozotocin
Peptide Fragments
Up-Regulation
Mice, Inbred C57BL
Disease Models, Animal
medicine.anatomical_structure
Endocrinology
Blood-Brain Barrier
cardiovascular system
business
human activities
Homeostasis
medicine.drug
Subjects
Details
- ISSN :
- 10982396
- Volume :
- 63
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Synapse (New York, N.Y.)
- Accession number :
- edsair.doi.dedup.....1eac4b3e31dd09bf9d8fd0d8fe73145f