Microfluorometry on antigen-antibody interaction in immunofluorescence using antigens covalently bound to agarose beads

Antigens covalently bound to agarose beads were found to be suitable for microfluorometry on individual beads of fluorescein-conjugated antibodies reacting with these antigens. With this technique, the interaction of conjugates with IgG, IgM and ovalbumin bound to the beads was studied. The system was found to be applicable to specificity tests. It also appeared to be more sensitive for high conjugate dilutions than immunofluorescence methods using tissue sections or cells of defined antigenicity as a substrate.