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Trials of the Automated Particle Counter for laboratory rearing of mosquito larvae
- Source :
- PLoS ONE, PLoS ONE, Vol 15, Iss 11, p e0241492 (2020)
- Publication Year :
- 2020
- Publisher :
- Public Library of Science, 2020.
-
Abstract
- As a means of obtaining reproducible and accurate numbers of larvae for laboratory rearing, we tested a large-particle flow-cytometer type device called the ‘Automated Particle Counter’ (APC). The APC is a gravity-fed, self-contained unit that detects changes in light intensity caused by larvae passing the detector in a water stream and controls dispensing by stopping the flow when the desired number has been reached. We determined the accuracy (number dispensed compared to the target value) and precision (distribution of number dispensed) of dispensing at a variety of counting sensitivity thresholds and larva throughput rates (larvae per second) using < 1-day old Anopheles gambiae and Aedes aegypti larvae. All measures were made using an APC algorithm called the ‘Smoothed Z-Score’ which allows the user to define how many standard deviations (Z scores) from the baseline light intensity a particle’s absorbance must exceed to register a count. We dispensed a target number of 100 An. gambiae larvae using Z scores from 2.5–8 and observed no difference among them in the numbers dispensed for scores from 2.5–6, however, scores of 7 and 8 under-counted (over-dispensed) larvae. Using a Z score ≤ 6, we determined the effect of throughput rate on the accuracy of the device to dispense An. gambiae larvae. For rates ≤ 98 larvae per second, the accuracy of dispensing a target of 100 larvae was - 2.29% ± 0.72 (95% CI of the mean) with a mode of 99 (49 of 348 samples). When using a Z score of 3.5 and rates ≤ 100 larvae per second, the accuracy of dispensing a target of 100 Ae. aegypti was - 2.43% ± 1.26 (95% CI of the mean) with a mode of 100 (6 of 42 samples). No effect on survival was observed on the number of An. gambiae first stage larvae that reached adulthood as a function of dispensing.
- Subjects :
- 0106 biological sciences
Life Cycles
Physiology
Anopheles gambiae
Eggs
Anopheles Gambiae
Disease Vectors
01 natural sciences
Mosquitoes
Automation
0302 clinical medicine
Larvae
Medical Conditions
Filter Paper
Mosquito larvae
Animal Cells
Reproductive Physiology
Medicine and Health Sciences
Mathematics
Flow Rate
Multidisciplinary
biology
Physics
Classical Mechanics
Eukaryota
Water stream
Flow Cytometry
Insects
Laboratory Equipment
Infectious Diseases
Larva
Physical Sciences
Medicine
Engineering and Technology
Cellular Types
Research Article
2019-20 coronavirus outbreak
animal structures
Arthropoda
Science
Immune Cells
030231 tropical medicine
Immunology
Antigen-Presenting Cells
Equipment
Aedes aegypti
Fluid Mechanics
Standard score
Aedes Aegypti
Continuum Mechanics
03 medical and health sciences
Animal science
parasitic diseases
Anopheles
Animals
Analysis of Variance
fungi
Organisms
Biology and Life Sciences
Fluid Dynamics
Cell Biology
biology.organism_classification
Invertebrates
Survival Analysis
Insect Vectors
010602 entomology
Light intensity
Species Interactions
Laboratories
Particle counter
Zoology
Entomology
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 15
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....1ee32a7e6ce60c20b6e598f0978037f9