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Effects of Calcium Hydroxide Intracanal Medications on T Helper (Th1, Th2, Th9, Th17, and Tfh) and Regulatory T (Treg) Cell Cytokines in Apical Periodontitis: A CONSORT RCT
- Source :
- Teixeira, F F C, Cardoso, F G R, Ferreira, N S, Corazza, B J M, Valera, M M C, Nascimento, G G & Martinho, F C 2022, ' Effects of Calcium Hydroxide Intracanal Medications on T Helper (Th1, Th2, Th9, Th17, and Tfh) and Regulatory T (Treg) Cell Cytokines in Apical Periodontitis : A CONSORT RCT ', Journal of Endodontics, vol. 48, no. 8, pp. 975-984 . https://doi.org/10.1016/j.joen.2022.04.010
- Publication Year :
- 2022
- Publisher :
- Elsevier BV, 2022.
-
Abstract
- Introduction: This Consolidated Standards of Reporting Trials randomized clinical trial investigated T helper (Th1, Th2, Th9, Th17, and Tfh) and regulatory T (Treg) cell–type cytokines and their networks in apical periodontitis (AP). We also assessed the effects of calcium hydroxide [Ca(OH)2] intracanal medications (ICMs) on helper T and Treg cell–type cytokines. Methods: Twenty teeth with primary endodontic infection and apical periodontitis were randomly divided into two groups: Ca(OH)2 + saline solution (n = 10) and Ca (OH)2 + 2% chlorhexidine-gel (n = 10). Samples were collected from the periradicular tissue fluid (PTF) before (PTFs1) and after 14 days of ICMs (PTFs2). The Human High Sensitivity T Cell Panel was used to quantify target T-helper (Th)1: interelukin (IL)-2, IL-12, and interferon-gamma (IFN-γ); Th2: IL-4, IL-5, and IL-13; Th9: IL-9; Th17: IL-17; T follicular helper cells (Tfh): IL-21; and Treg-cell-type cytokine: IL-10. Results: Th1-type cytokines were higher than Th2-type ones, at PTFs1. Positive (+) associations were found among all Th1-type cytokines and all Th2-type cytokines. There were negative (-) correlations between all Th1- and Th2-type cytokines. Size of radiolucent lesions and symptoms (tenderness to percussion and/or pain on palpation) were positively correlated with Th1-type cytokines, IL-17, and IL-21 but negatively correlated with Th2-type cytokines and IL-10 (all, P .05). Conclusions: Complex T-cell cytokine networks are involved in AP. Both Ca(OH)2 ICMs effectively increased IL-4, IL-5, IL-10, and IL-13 and lowered IL-2, IL-12, IL-17, IL-21, and IFN-γ.
- Subjects :
- Interleukin-13
T Follicular Helper Cells
Interleukin-17
intracanal medication
Th1 Cells
root canal
Interleukin-12
T-Lymphocytes, Regulatory
Interleukin-10
Calcium Hydroxide
Interferon-gamma
endodontics
Th2 Cells
inflammation
T-helper
Humans
Th17 Cells
Cytokines
Interleukin-4
Interleukin-5
General Dentistry
Periapical Periodontitis
Subjects
Details
- ISSN :
- 00992399
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Journal of Endodontics
- Accession number :
- edsair.doi.dedup.....1f0222e55a5c81f0ccbe8feeb09716f8