Back to Search Start Over

High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation

Authors :
Martin Moche
Tetsuro Kokubo
Maria Sunnerhagen
Yoshifumi Ohyama
Mitsuhiko Ikura
Marina I. Siponen
Madhanagopal Anandapadamanaban
Cecilia Andrésen
Sara Helander
Patrik Lundström
Source :
Nature structural & molecular biology
Publication Year :
2013

Abstract

The general transcription factor TFIID provides a regulatory platform for transcription initiation. Here we present the crystal structure (1.97 Å) and NMR analysis of yeast TAF1 N-terminal domains TAND1 and TAND2 when bound to yeast TBP, together with mutational data. The yTAF1-TAND1, which in itself acts as a transcriptional activator, binds into the DNA-binding TBP concave surface by presenting similar anchor residues to TBP as E. coli Mot1 but from a distinct structural scaffold. Furthermore, we show how yTAF1-TAND2 employs an aromatic and acidic anchoring pattern to bind a conserved yTBP surface groove traversing the basic helix region, and we find highly similar TBP-binding motifs also presented by the structurally distinct TFIIA, Mot1 and Brf1 proteins. Our identification of these anchoring patterns, which can be easily disrupted or enhanced, provides compelling insight into the competitive multiprotein TBP interplay critical to transcriptional regulation.

Details

Language :
English
ISSN :
15459985 and 15459993
Volume :
20
Issue :
8
Database :
OpenAIRE
Journal :
Nature structural & molecular biology
Accession number :
edsair.doi.dedup.....1f0839a1e65104fd6dee172f9086a485