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High-resolution structure of TBP with TAF1 reveals anchoring patterns in transcriptional regulation
- Source :
- Nature structural & molecular biology
- Publication Year :
- 2013
-
Abstract
- The general transcription factor TFIID provides a regulatory platform for transcription initiation. Here we present the crystal structure (1.97 Å) and NMR analysis of yeast TAF1 N-terminal domains TAND1 and TAND2 when bound to yeast TBP, together with mutational data. The yTAF1-TAND1, which in itself acts as a transcriptional activator, binds into the DNA-binding TBP concave surface by presenting similar anchor residues to TBP as E. coli Mot1 but from a distinct structural scaffold. Furthermore, we show how yTAF1-TAND2 employs an aromatic and acidic anchoring pattern to bind a conserved yTBP surface groove traversing the basic helix region, and we find highly similar TBP-binding motifs also presented by the structurally distinct TFIIA, Mot1 and Brf1 proteins. Our identification of these anchoring patterns, which can be easily disrupted or enhanced, provides compelling insight into the competitive multiprotein TBP interplay critical to transcriptional regulation.
- Subjects :
- Models, Molecular
Saccharomyces cerevisiae Proteins
Transcription, Genetic
Protein Conformation
genetic processes
macromolecular substances
Biology
Article
03 medical and health sciences
0302 clinical medicine
Structural Biology
Transcription (biology)
Transcription Factor TFIIIB
Transcriptional regulation
Molecular Biology
030304 developmental biology
TATA-Binding Protein Associated Factors
Adenosine Triphosphatases
0303 health sciences
General transcription factor
TATA-Box Binding Protein
Cell biology
Crystallography
TAF1
Gene Expression Regulation
Transcription Factor TFIID
health occupations
Transcription factor II D
Crystallization
030217 neurology & neurosurgery
Protein Binding
Subjects
Details
- Language :
- English
- ISSN :
- 15459985 and 15459993
- Volume :
- 20
- Issue :
- 8
- Database :
- OpenAIRE
- Journal :
- Nature structural & molecular biology
- Accession number :
- edsair.doi.dedup.....1f0839a1e65104fd6dee172f9086a485