Improvements in production of cDNA libraries in lambda gtll. Detection of ligation inhibitor and separation of cDNA from free linkers

A ligation assay was designed to determine if a ligation inhibitor is present in a Bio-Gel A-50-m column, the traditional tool used for separation of cDNA from free linkers during the lambda gtll cloning procedure. A new and simple electrophoresis method is also presented for performing this separation, which is rapid, efficient, and reduces the chance of carrying along a ligation inhibitor.