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Dietary omega-3 PUFA improved tubular function after ischemia induced acute kidney injury in mice but did not attenuate impairment of renal function
- Source :
- Prostaglandins and Other Lipid Mediators, Prostaglandins and Other Lipid Mediators, Elsevier, 2019, pp.106386. ⟨10.1016/j.prostaglandins.2019.106386⟩
- Publication Year :
- 2019
- Publisher :
- HAL CCSD, 2019.
-
Abstract
- Background Acute kidney injury (AKI) is an important complication after major surgery and solid organ transplantation. Here, we present a dietary omega-3 polyunsaturated fatty acid (n3-PUFA) supplementation study to investigate whether pre-treatment can reduce ischemia induced AKI in mice. Methods Male 12–14 week old C57BL/6 J mice received a linoleic acid rich sunflower oil based standard diet containing 10 % fat (STD) or the same diet enriched with n3-PUFA (containing 1 % EPA and 1 % DHA) (STD + n3). After 14 days of feeding bilateral 30 min renal ischemia reperfusion injury (IRI) was conducted to induce AKI and mice were sacrificed at 24 h. Serum creatinine and blood urea nitrogen (BUN) as well as liver enzyme elevation were measured. Kidney damage was analyzed by histology and immunohistochemistry. Furthermore, pro-inflammatory cytokines (IL-6, MCP-1) were determined by qPCR. FA and oxylipin pattern were quantified in blood and kidneys by GC-FID and LC–MS/MS, respectively. Results n3-PUFA supplementation prior to renal IRI increased systemic and renal levels of n3-PUFA. Consistently, eicosanoids and other oxylipins derived from n3-PUFA including precursors of specialized pro-resolving mediators were elevated while n6-PUFA derived mediators such as pro-inflammatory prostaglandins were decreased. Feeding of n3-PUFA did not attenuate renal function impairment, morphological renal damage and inflammation characterized by IL-6 and MCP-1 elevation or neutrophil infiltration. However, the tubular transport marker alpha-1 microglobulin (A1M) was significantly higher expressed in proximal tubular epithelial cells of STD + n3 compared to STD fed mice. This indicates a better integrity of proximal tubular epithelial cells and thus significant protection of tubular function. In addition, heme oxygenase-1 (HO-1) which protects tubular function was also up-regulated in the treatment group receiving n3-PUFA supplemented chow. Discussion We showed that n3-PUFA pre-treatment did not affect overall renal function or renal inflammation in a mouse model of moderate ischemia induced AKI, but tubular transport was improved. In conclusion, dietary n3-PUFA supplementation altered the oxylipin levels significantly but did not protect from renal function deterioration or attenuate ischemia induced renal inflammation.
- Subjects :
- 0301 basic medicine
Male
medicine.medical_specialty
Physiology
[SDV]Life Sciences [q-bio]
Ischemia
Renal function
Inflammation
Mice, Transgenic
030204 cardiovascular system & hematology
urologic and male genital diseases
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Internal medicine
Fatty Acids, Omega-3
Medicine
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
Blood urea nitrogen
ComputingMilieux_MISCELLANEOUS
2. Zero hunger
Pharmacology
Creatinine
Kidney
business.industry
Beta-2 microglobulin
Acute kidney injury
Cell Biology
Acute Kidney Injury
medicine.disease
3. Good health
030104 developmental biology
Endocrinology
medicine.anatomical_structure
Kidney Tubules
chemistry
Reperfusion Injury
medicine.symptom
business
Subjects
Details
- Language :
- English
- ISSN :
- 10988823
- Database :
- OpenAIRE
- Journal :
- Prostaglandins and Other Lipid Mediators, Prostaglandins and Other Lipid Mediators, Elsevier, 2019, pp.106386. ⟨10.1016/j.prostaglandins.2019.106386⟩
- Accession number :
- edsair.doi.dedup.....1f2f43c4765488d12c246f85e82c8fe8