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Genetic and Molecular Analysis of Root Hair Development in Arabis alpina

Authors :
Andrea Schrader
Korbinian Schneeberger
Mona Mapar
Maria C. Albani
Lisa Stephan
George Coupland
Divykriti Chopra
Hequan Sun
Martin Hülskamp
Source :
Frontiers in Plant Science, Vol 12 (2021), Frontiers in Plant Science
Publication Year :
2021
Publisher :
Frontiers Media S.A., 2021.

Abstract

Root hair formation in Arabidopsis thaliana is a well-established model system for epidermal patterning and morphogenesis in plants. Over the last decades, many underlying regulatory genes and well-established networks have been identified by thorough genetic and molecular analysis. In this study, we used a forward genetic approach to identify genes involved in root hair development in Arabis alpina, a related crucifer species that diverged from A. thaliana approximately 26–40 million years ago. We found all root hair mutant classes known in A. thaliana and identified orthologous regulatory genes by whole-genome or candidate gene sequencing. Our findings indicate that the gene-phenotype relationships regulating root hair development are largely conserved between A. thaliana and A. alpina. Concordantly, a detailed analysis of one mutant with multiple hairs originating from one cell suggested that a mutation in the SUPERCENTIPEDE1 (SCN1) gene is causal for the phenotype and that AaSCN1 is fully functional in A. thaliana. Interestingly, we also found differences in the regulation of root hair differentiation and morphogenesis between the species, and a subset of root hair mutants could not be explained by mutations in orthologs of known genes from A. thaliana. This analysis provides insight into the conservation and divergence of root hair regulation in the Brassicaceae.

Details

Language :
English
Volume :
12
Database :
OpenAIRE
Journal :
Frontiers in Plant Science
Accession number :
edsair.doi.dedup.....1f3939c62b307ed1d5ac9d1822555551
Full Text :
https://doi.org/10.3389/fpls.2021.767772/full