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Porous Nanohydroxyapatite/Collagen Scaffolds Loading Insulin PLGA Particles for Restoration of Critical Size Bone Defect
- Source :
- ACS applied materialsinterfaces. 9(13)
- Publication Year :
- 2017
-
Abstract
- Insulin is considered to be a classical central regulator of energy homeostasis. Recently, the effect of insulin on bone has gained a lot of attention, but little attention has been paid to the application in bone tissue engineering. In this study, porous nanohydroxyapatite/collagen (nHAC) scaffolds incorporating poly lactic-co-glycolic acid (PLGA) particles were successfully developed as an insulin delivery platform for bone regeneration. Bioactive insulin was successfully released from the PLGA particles within the scaffold, and the size of the particles as well as the release kinetics of the insulin could be efficiently controlled through Shirasu porous glass premix membrane emulsification technology. It was indicated that the nHAC/PLGA composite scaffolds possessed favorable mechanical and structural properties for cell adhesion and proliferation, as well as the differentiation into osteoblasts. It was also demonstrated that the nHAC/PLGA scaffolds implanted into a rabbit critical-size mandible defect possessed tissue compatibility and higher bone restoration capacity compared with the defects that were filled with or without nHAC scaffolds. Furthermore, the in vivo results showed that the nHAC/PLGA scaffolds which incorporated insulin-loaded microspheres with a size of 1.61 μm significantly accelerated bone healing compared with two other composite scaffolds. Our study indicated that the local insulin released at the optimal time could substantially and reproducibly improve bone repair.
- Subjects :
- 0301 basic medicine
Scaffold
Materials science
Bone Regeneration
medicine.medical_treatment
Kinetics
02 engineering and technology
Porous glass
03 medical and health sciences
chemistry.chemical_compound
Glycols
medicine
Animals
Insulin
General Materials Science
Lactic Acid
Composite material
Cell adhesion
Membrane emulsification
Bone regeneration
Tissue Engineering
Tissue Scaffolds
technology, industry, and agriculture
021001 nanoscience & nanotechnology
Nanostructures
PLGA
030104 developmental biology
chemistry
Collagen
Rabbits
0210 nano-technology
Porosity
Polyglycolic Acid
Biomedical engineering
Subjects
Details
- ISSN :
- 19448252
- Volume :
- 9
- Issue :
- 13
- Database :
- OpenAIRE
- Journal :
- ACS applied materialsinterfaces
- Accession number :
- edsair.doi.dedup.....1f5c447d34bbaf183facde2051e16e26