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Kinetics and Interplay of Mediators of Inflammation-Induced Bone Damage in the Course of Adjuvant Arthritis
- Source :
- International Journal of Immunopathology and Pharmacology. 26:37-48
- Publication Year :
- 2013
- Publisher :
- SAGE Publications, 2013.
-
Abstract
- Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammation, bone erosion, and cartilage destruction in the joints. It is increasingly being realized that inflammation might play an important role in inducing bone damage in arthritis. However, there is limited validation of this concept in vivo in well-controlled experimental conditions. We addressed this issue using the adjuvant arthritis (AA) model of RA. In AA, the draining lymph nodes are the main sites of activation of pathogenic leukocytes, which then migrate into the joints leading to the induction of arthritis. We tested the temporal kinetics of mediators of bone damage [e.g., receptor activator of nuclear factor kappa-B ligand (RANKL), osteoprotegerin (OPG) and osteopontin (OPN)] and inflammation (pro-inflammatory cytokines and chemokines) in the draining lymph node cells (LNC) at different phases of AA, and then examined their inter-relationships. Our study revealed that, together with cytokines/chemokines, some of the mediators of bone remodeling are also produced in LNC. Various cytokines/chemokines showed distinct kinetics of expression as well as patterns of correlation with mediators of bone remodeling at different phases of the disease. Pro-inflammatory cytokines such as TNF-α are known to play an important role in bone damage. Interestingly, there was a positive correlation between TNF-α and RANKL, between RANKL and each of the 3 chemokines tested (RANTES, MIP-1α, and GRO/KC), and between TNF-α and RANTES. Our results in the AA model lend support to the concept of osteo-immune crosstalk during the course of autoimmune arthritis.
- Subjects :
- Male
musculoskeletal diseases
Chemokine
Immunology
Arthritis
Inflammation
Article
Bone remodeling
Osteoprotegerin
medicine
Animals
Immunology and Allergy
Osteopontin
Cells, Cultured
Pharmacology
biology
business.industry
medicine.disease
Arthritis, Experimental
Rats
Kinetics
Rats, Inbred Lew
RANKL
Rheumatoid arthritis
biology.protein
Cytokines
Bone Remodeling
Lymph Nodes
medicine.symptom
business
Subjects
Details
- ISSN :
- 20587384
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- International Journal of Immunopathology and Pharmacology
- Accession number :
- edsair.doi.dedup.....1f7ab16567cbfd65cb3e54d96be854e1
- Full Text :
- https://doi.org/10.1177/039463201302600104