A rapid procedure for the separation of amino-acids on paper chromatograms

IN this laboratory the technique of circular paper chromatography has been modified in that small rectangular strips of filter paper are used rather than filter paper disks. This has resulted in the separation of compounds as round compact spots rather than circular bands. In addition, a combination of a horizontal solvent flow with an increase in developing temperature has resulted in a rapid increase in the mobility of the developing solvent coupled with a desirable increase in resolution.