Back to Search
Start Over
Integrin alpha v beta 3 differentially regulates adhesive and phagocytic functions of the fibronectin receptor alpha 5 beta 1
- Source :
- The Journal of Cell Biology
- Publication Year :
- 1994
-
Abstract
- The plasma protein fibronectin is an important opsonin in wound repair and host defense. To better understand the process of fibronectin-mediated phagocytosis, we have transfected K562 cells, which endogenously express alpha 5 beta 1, with alpha v beta 3. In these transfectants, antibodies to alpha v beta 3 block phagocytosis of fibronectin-opsonized beads completely, even though half the ingestion occurs through endogenous alpha 5 beta 1 receptors. alpha 5 beta 1-mediated adhesion to fibronectin-coated surfaces is unaffected by alpha v beta 3 ligation. Neither alpha v beta 5 nor alpha M beta 2 ligation affects alpha 5 beta 1 phagocytic function in transfectants expressing these receptors. Pharmacologic data suggest that alpha v beta 3 ligation suppresses the phagocytic competence of high affinity alpha 5 beta 1 receptors through a signal transduction pathway, perhaps involving protein kinase C. In addition to its significance for phagocytosis, alpha v beta 3 regulation of alpha 5 beta 1 function may be significant for its roles in cell migration, metastasis, and angiogenesis.
- Subjects :
- Integrins
Alpha-v beta-3
Alpha-v beta-5
Phagocytosis
Lactams, Macrocyclic
Integrin
Alpha (ethology)
Biology
Naphthalenes
Receptors, Cytoadhesin
Transfection
Piperazines
Cell Line
chemistry.chemical_compound
Receptors, Fibronectin
1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
Benzoquinones
Cell Adhesion
Tumor Cells, Cultured
Humans
Polycyclic Compounds
Receptors, Vitronectin
Cloning, Molecular
Protein kinase A
Beta (finance)
Receptor
Protein Kinase Inhibitors
Protein Kinase C
Quinones
Cell Biology
Articles
Protein-Tyrosine Kinases
Flow Cytometry
Isoquinolines
Molecular biology
Genistein
Isoflavones
Fibronectins
Kinetics
chemistry
Rifabutin
biology.protein
Leukemia, Erythroblastic, Acute
Signal Transduction
Subjects
Details
- ISSN :
- 00219525
- Volume :
- 127
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- The Journal of cell biology
- Accession number :
- edsair.doi.dedup.....1fc0df8de45190d6c6749e77b79a5416