Back to Search
Start Over
Binding Properties, Cell Delivery, and Gene Transfer of Adenoviral Penton Base Displaying Bacteriophage
Binding Properties, Cell Delivery, and Gene Transfer of Adenoviral Penton Base Displaying Bacteriophage
- Source :
- Virology. 282:102-112
- Publication Year :
- 2001
- Publisher :
- Elsevier BV, 2001.
-
Abstract
- The penton base of adenovirus mediates viral attachment to integrin receptors and particle internalisation, properties that can be exploited to reengineer prokaryotic viruses for the infection of mammalian cells. We report that filamentous phage displaying either the full-length penton base gene or a central region of 107 amino acids on their surface were able to bind, internalise, and transduce mammalian cells expressing integrin receptors. Both phage bound alphavbeta3, alphavbeta5, alpha3beta1, and alpha5beta1 integrin subtypes. Cell-binding was shown by electron microscopy; internalisation was investigated by immunofluorescence and confirmed by micropanning. As it has been described for adenovirus, pharmacologic disruption of phosphoinositide-30H kinase, but not of myosin light-chain kinase, inhibited phage internalisation. Recombinant phage encoding an eukaryotic expression cassette was able to mediate gene expression in mammalian cells. Taken together, these data open insights for the exploit of recombinant phage for integrin-targeted gene delivery.
- Subjects :
- Integrins
Phage display
viruses
Phagemid
Genetic Vectors
Integrin
Fluorescent Antibody Technique
Gene delivery
Adenoviridae
law.invention
Bacteriophage
Phosphatidylinositol 3-Kinases
Capsid
endocytosis
gene therapy
integrins
phage-display
Transduction, Genetic
law
Virology
Gene expression
Humans
Bacteriophages
Gene
biology
biology.organism_classification
Molecular biology
Microscopy, Electron
Recombinant DNA
biology.protein
Capsid Proteins
Carrier Proteins
Oligopeptides
HeLa Cells
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 282
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....1fda4925d9ce2f39056269f2c1d50862
- Full Text :
- https://doi.org/10.1006/viro.2000.0809