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Human Serum Albumin-Occupying-Based Fluorescence Turn-On Analysis of Antiepileptic Drug Tiagabine Hydrochloride
- Source :
- Analytical Chemistry. 92:3555-3562
- Publication Year :
- 2020
- Publisher :
- American Chemical Society (ACS), 2020.
-
Abstract
- Tiagabine hydrochloride (TGB) is a clinically frequently used drug for anticonvulsion and reducing epileptic frequency. Over administration of TGB could bring about adverse effects, such as speech disorder, depression, and even suicidal tendencies. Therefore, accessible and sensitive assay for analysis of TGB becomes an urgent need toward guiding clinical medication. Here, we present the first report on fluorescence turn-on detection of TGB in urine testing. In this protocol, a fluorescent dye, perylene tetracarboxylic acid imide derivative (PTAI), is found specifically occupying the Sudlow site II of human serum albumin (HSA) and displays a new phenomenon of binding-induced quenching (BIQ). In presence of TGB, competitive binding of the TGB to the site II of HSA will trigger release of PTAI, thus successfully lighting up the fluorescence of PTAI. This label-free assay enjoys a broader working range (1-350 μM) and lower detection limit (0.218 μM) than the traditional liquid chromatography method and is uninterfered by the miscellaneous in the artificial urine. The BIQ probe highlights the merits of HSA as a quencher and a molecular recognition unit, and it opens up a way for studying drug-HSA interaction mechanism and noninvasive pharmaceutical testing.
- Subjects :
- Models, Molecular
Drug
Protein Conformation
media_common.quotation_subject
Serum albumin
Serum Albumin, Human
Biosensing Techniques
Buffers
Analytical Chemistry
chemistry.chemical_compound
Molecular recognition
medicine
Humans
Tiagabine
Imide
media_common
Chromatography
Quenching (fluorescence)
biology
Human serum albumin
Fluorescence
Spectrometry, Fluorescence
chemistry
biology.protein
Anticonvulsants
Perylene
medicine.drug
Subjects
Details
- ISSN :
- 15206882 and 00032700
- Volume :
- 92
- Database :
- OpenAIRE
- Journal :
- Analytical Chemistry
- Accession number :
- edsair.doi.dedup.....1ff13c9773b107ea5146ac0118e1d7fa