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Uncoating of COPII from ER exit site membranes precedes cargo accumulation and membrane fission

Authors :
Yoji Yonemura
Eitan Erez Zahavi
Anna Mironov
Alexander A. Mironov
Adva Yeheskel
Ella H. Sklan
Galina V. Beznoussenko
Inbar Nevo-Yassaf
Anna Dukhovny
Koret Hirschberg
Tamar Aviad
Christoph Kaether
Yakey Yaffe
Ilya Brodsky
Olga Shomron
George H. Patterson
Metsada Pasmanik-Chor
Eran Perlson
Publication Year :
2019
Publisher :
Cold Spring Harbor Laboratory, 2019.

Abstract

SummaryCOPII and COPI are considered to be analogous sets of vesicle coat protein heterocomplexes. Coupled to cargo selection, they mediate the formation of membrane vesicles translocating in opposite directions to differ rent destinations within the secretory pathway. Here, live cell and electron microscopy provided evidence for a different localization and mode of function of the COPII coat during protein export from the endoplasmic reticulum (ER). Pharmaceutical and genetic perturbations of ER-Golgi transport were used to demonstrate that COPII is recruited to membranes defining the boundary of ER-ER Exit Sites (ERES) where it facilitates selective cargo concentration. Uncoating of COPII membranes precedes cargo accumulation and fission of Golgi-bound carriers. Moreover, we report what may be direct transfer of cargo to the Golgi apparatus from Golgi-associated BFA sensitive ERESs. Finally, in ldlF cells the stably expressed functional ε-COPI-EYFP labeled both ERESs and anterograde carriers. These findings change our understanding of the role of coat proteins in ER to Golgi transport.

Details

Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....200097a6cf1038d2bc0f2340fe735dfa
Full Text :
https://doi.org/10.1101/727107