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Porphyromonas gingivalis lipopolysaccharide affects oral epithelial connections via pyroptosis
- Source :
- Journal of Dental Sciences, Vol 16, Iss 4, Pp 1255-1263 (2021), Journal of Dental Sciences
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- Background/purpose: Pyroptosis is a form of programmed cell death dependent on the activation of caspase-1. Porphyromonas gingivalis (P. gingivalis) is a major pathogenic bacterium in periodontitis and its lipopolysaccharide (LPS) can trigger inflammation. However, whether P. gingivalis-LPS affects epithelial connections or triggers pyroptosis in the gingival epithelium is unknown. Materials and methods: Gingival samples from human donors were collected and the expression levels of E-cadherin, nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3), caspase-1/4/5, interleukin (IL)-18, and IL-1β were examined. P. gingivalis-LPS was injected into rat gingival sulcus to establish gingivitis models, and the expression levels of E-cadherin, NLRP3, caspase-1/11, IL-18, and IL-1β were compared via immunohistochemistry. The mRNA levels of E-cadherin, caspase-1, IL-18, and IL-1β were evaluated in oral mucosa epithelial cells (OMECs) and rat gingival tissues. Results: In the present study, NLRP3 (p
- Subjects :
- Lipopolysaccharide
Inflammation
Microbiology
03 medical and health sciences
chemistry.chemical_compound
Gingivitis
0302 clinical medicine
medicine
Pyroptosis
Gingival sulcus
General Dentistry
Porphyromonas gingivalis
Periodontitis
biology
Chemistry
Interleukin
Epithelial connection
RK1-715
030206 dentistry
medicine.disease
biology.organism_classification
030220 oncology & carcinogenesis
Dentistry
Original Article
medicine.symptom
Subjects
Details
- Language :
- English
- ISSN :
- 19917902
- Volume :
- 16
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Journal of Dental Sciences
- Accession number :
- edsair.doi.dedup.....2026dca5daa5c61f3f059c3e7b350470