Back to Search
Start Over
Functional roles of cadherin, aminopeptidase-N and alkaline phosphatase from Helicoverpa armigera (Hübner) in the action mechanism of Bacillus thuringiensis Cry2Aa
- Source :
- Scientific Reports
- Publication Year :
- 2017
- Publisher :
- Springer Science and Business Media LLC, 2017.
-
Abstract
- A pyramid strategy combining the Cry1A and Cry2A toxins in Bt crops has been widely used throughout the world to delay pest adaption to transgenic crops and broaden the insecticidal spectrum. Midgut membrane-bound cadherin (CAD), aminopeptidase-N (APN) and alkaline phosphatase (ALP) are important for Cry1A toxicity in some lepidopteran larvae, but the proteins that bind Cry2A in the midgut of target insects and their role in the Cry2A mechanism of action are still unclear. In this study, we found that heterologously expressed CAD, APN4 and ALP2 peptides from the midgut of Helicoverpa armigera could bind to the Cry2Aa toxin with a high affinity. Additionally, the efficiency of Cry2Aa insecticidal activity against H. armigera larvae was obviously reduced after the genes encoding these proteins were silenced with specific siRNAs: CAD- and ALP2-silenced larvae showed significantly similar reductions in mortality due to the Cry2Aa toxin (41.67% and 43.06%, respectively), whereas a larger reduction in mortality was observed in APN4-silenced larvae (61.11%) than in controls. These results suggest that CAD, APN4 and ALP2 are involved in the mechanism of action of Cry2Aa in H. armigera and may play important functional roles in the toxicity of the Cry2Aa toxin.
- Subjects :
- 0301 basic medicine
Insecticides
030106 microbiology
Bacillus thuringiensis
CD13 Antigens
Moths
Helicoverpa armigera
Aminopeptidase
Article
Hemolysin Proteins
03 medical and health sciences
Bacterial Proteins
medicine
Animals
Mode of action
Multidisciplinary
Bacillus thuringiensis Toxins
biology
Cadherin
fungi
Midgut
Alkaline Phosphatase
Cadherins
biology.organism_classification
Endotoxins
Intestines
030104 developmental biology
Mechanism of action
Biochemistry
Larva
Insect Proteins
Alkaline phosphatase
medicine.symptom
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....2040bdd22f2979c03b19f818f6d9a470