Back to Search
Start Over
Considerations for quality assurance of multiplex malaria antigen detection assays with large sample sets
- Source :
- Scientific Reports, Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Multiplex assays for malaria antigen detection can gather data from large sample sets, but considerations for the consistency and quality assurance (QA) of mass testing lack evaluation. We present a QA framework for a study occurring November 2019 to March 2020 involving 504 assay plates detecting four Plasmodium antigens: pan-Plasmodium aldolase and lactate dehydrogenase (LDH), histidine-rich protein 2 (HRP2), P. vivax LDH (PvLDH). Controls on each plate included buffer blank, antigen negative blood, and 4-point positive dilution curve. The blank and negative blood provided consistently low signal for all targets except for pAldolase, which showed variability. Positive curve signals decreased throughout the 5-month study duration but retained a coefficient of variation (CV) of
- Subjects :
- Quality Control
Plasmodium
Adolescent
Science
Coefficient of variation
030231 tropical medicine
Protozoan Proteins
Nigeria
Antigens, Protozoan
Policy and public health in microbiology
Article
Standard deviation
Immunological techniques
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Antigen
Fructose-Bisphosphate Aldolase
Lactate dehydrogenase
Humans
Medicine
Serologic Tests
Malaria antigen
Multiplex
030212 general & internal medicine
Child
Multidisciplinary
Chromatography
L-Lactate Dehydrogenase
business.industry
Malaria
Large sample
chemistry
Parasitology
business
Microbiology techniques
Quality assurance
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....209a8958cb423855f87ae0fb0a771778