Loop-mediated isothermal amplification assay for detection of Coxiella burnetii targeting the com1 gene

Authors :: Jay Prakash Yadav
Satya Veer Singh Malik
Sukhadeo B. Barbuddhe
Durga Prasad Das
Deepak B. Rawool
Radhakrishna Sahu
Source :: Journal of Microbiological Methods. 155:55-58
Publication Year :: 2018
Publisher :: Elsevier BV, 2018.
Abstract: We developed the com1 gene based loop-mediated isothermal amplification (LAMP) assay for the detection of Coxiella burnetii and validated it by screening DNA isolated from serum samples collected from animals and humans. The detection of Coxiella by LAMP assay was comparable with the com1 based-PCR.

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