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Procedure for controlling number of repeats, orientation, and order during cloning of oligonucleotides
- Source :
- BioTechniques. 36:933-936
- Publication Year :
- 2004
- Publisher :
- Future Science Ltd, 2004.
-
Abstract
- COOL Cloning Insertion of short DNA sequences into plasmids is a widely applied technique, but it can be complicated by lack of precision and the need to screen and separate clones in order to iden...
- Subjects :
- DNA, Bacterial
Cloning
Genetics
Orientation (computer vision)
Oligonucleotide
Oligonucleotides
food and beverages
Biology
General Biochemistry, Genetics and Molecular Biology
DNA sequencing
Order (biology)
Plasmid
Tandem Repeat Sequences
Escherichia coli
Mutagenesis, Site-Directed
Cloning, Molecular
Genetic Engineering
Biotechnology
Subjects
Details
- ISSN :
- 19409818 and 07366205
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- BioTechniques
- Accession number :
- edsair.doi.dedup.....20b9bec2a5f6e8c609602384045cfd44
- Full Text :
- https://doi.org/10.2144/04366bm02