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Cloning and sequencing of a cell surface protein-encoding gene conserved in sea urchin species
- Source :
- Gene. 141:243-248
- Publication Year :
- 1994
- Publisher :
- Elsevier BV, 1994.
-
Abstract
- We report the nucleotide sequence of a fragment of DNA derived from a sea urchin genomic clone containing the cell surface Bep4 (butanol-extracted protein 4)-encoding gene. The structural gene is interrupted by four introns and the promoter region contains TATA and CAAT consensus motifs. The transcription start point (tsp) was also determined. Remarkable homologies, between Bep4 and other proteins known to be involved in cell interactions, were observed regarding two poteintial Ca2+-binding sites and the corresponding DNA consensus sequences. We also report the conservation of the bep4 gene and its corresponding Bep4 protein between various sea urchin species by way of Southern and Western blotting.
- Subjects :
- Molecular Sequence Data
Paracentrotus lividus
biology.animal
Consensus Sequence
Genetics
Consensus sequence
Animals
Amino Acid Sequence
Cloning, Molecular
Peptide Chain Initiation, Translational
Promoter Regions, Genetic
Gene
Sea urchin
Base Sequence
Sequence Homology, Amino Acid
biology
Structural gene
Intron
Nucleic acid sequence
Membrane Proteins
Promoter
General Medicine
biology.organism_classification
TATA Box
Genes
Sea Urchins
Subjects
Details
- ISSN :
- 03781119
- Volume :
- 141
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....20be5545cfa549c701f76ac93b509bd8